1nxk: Difference between revisions

Revision as of 03:05, 3 May 2008

Crystal structure of staurosporine bound to MAP KAP kinase 2

OverviewOverview

MAP KAP kinase 2 (MK2), a Ser/Thr kinase, plays a crucial role in the inflammatory process. We have determined the crystal structures of a catalytically active C-terminal deletion form of human MK2, residues 41-364, in complex with staurosporine at 2.7 A and with ADP at 3.2 A, revealing overall structural similarity with other Ser/Thr kinases. Kinetic analysis reveals that the K(m) for ATP is very similar for MK2 41-364 and p38-activated MK2 41-400. Conversely, the catalytic rate and binding for peptide substrate are dramatically reduced in MK2 41-364. However, phosphorylation of MK2 41-364 by p38 restores the V(max) and K(m) for peptide substrate to values comparable to those seen in p38-activated MK2 41-400, suggesting a mechanism for regulation of enzyme activity.

About this StructureAbout this Structure

1NXK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Catalytically active MAP KAP kinase 2 structures in complex with staurosporine and ADP reveal differences with the autoinhibited enzyme., Underwood KW, Parris KD, Federico E, Mosyak L, Czerwinski RM, Shane T, Taylor M, Svenson K, Liu Y, Hsiao CL, Wolfrom S, Maguire M, Malakian K, Telliez JB, Lin LL, Kriz RW, Seehra J, Somers WS, Stahl ML, Structure. 2003 Jun;11(6):627-36. PMID:12791252 Page seeded by OCA on Sat May 3 03:05:56 2008

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OCA

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 [[Image:1nxk.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1nxk", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|GENE= MAPKAPK2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_1nxk|  PDB=1nxk  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nxk OCA], [http://www.ebi.ac.uk/pdbsum/1nxk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nxk RCSB]</span>
-}}
 '''Crystal structure of staurosporine bound to MAP KAP kinase 2'''
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 [[Category: Underwood, K W.]]
 [[Category: Wolfrom, S.]]
-[[Category: mk2]]
+[[Category: Mk2]]
-[[Category: phosphorylation]]
+[[Category: Phosphorylation]]
-[[Category: protein kinase]]
+[[Category: Protein kinase]]
-[[Category: staurosporine]]
+[[Category: Staurosporine]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:05:56 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:36:59 2008''