1nrj: Difference between revisions

Revision as of 02:53, 3 May 2008

Signal Recognition Particle Receptor Beta-Subunit in Complex with the SRX Domain from the Alpha-Subunit

OverviewOverview

Protein translocation across and insertion into membranes is a process essential to all life forms. In higher eukaryotes, this process is initiated by targeting the translating ribosome to the endoplasmic reticulum via the signal recognition particle (SRP) and its membrane-associated heterodimeric receptor (SR). This targeting step is regulated by three G proteins, SRP54, SR alpha, and SR beta, which act in concert. Little is known about the regulatory role of SR beta. Here, we present the 1.7 A crystal structure of the SR beta-GTP subunit in complex with the interaction domain of SR alpha. Strikingly, the binding interface overlaps largely with the switch 1 region of SR beta. This finding, together with additional biochemical data, shows that the eukaryotic SR is a conditional and not an obligate heterodimer. The results suggest that the GTP/GDP switch cycle of SR beta functions as a regulatory switch for the receptor dimerization. We discuss the implications for the translocation pathway.

About this StructureAbout this Structure

1NRJ is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the function of the beta subunit of the eukaryotic signal recognition particle receptor., Schwartz T, Blobel G, Cell. 2003 Mar 21;112(6):793-803. PMID:12654246 Page seeded by OCA on Sat May 3 02:53:43 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1nrj.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1nrj |SIZE=350|CAPTION= <scene name='initialview01'>1nrj</scene>, resolution 1.70&Aring;
+The line below this paragraph, containing "STRUCTURE_1nrj", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GTP:GUANOSINE-5&#39;-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= SRP101 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRP102 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_1nrj|  PDB=1nrj  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nrj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nrj OCA], [http://www.ebi.ac.uk/pdbsum/1nrj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nrj RCSB]</span>
-}}
 '''Signal Recognition Particle Receptor Beta-Subunit in Complex with the SRX Domain from the Alpha-Subunit'''
@@ Line 26: / Line 23: @@
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Schwartz, T.]]
-[[Category: endoplasmic reticulum]]
+[[Category: Endoplasmic reticulum]]
-[[Category: gtp-binding]]
+[[Category: Gtp-binding]]
-[[Category: gtpase-effector complex]]
+[[Category: Gtpase-effector complex]]
-[[Category: receptor]]
+[[Category: Receptor]]
-[[Category: signal recognition particle]]
+[[Category: Signal recognition particle]]
-[[Category: transmembrane]]
+[[Category: Transmembrane]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:53:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:34:28 2008''