1nqd: Difference between revisions

Revision as of 02:51, 3 May 2008

CRYSTAL STRUCTURE OF CLOSTRIDIUM HISTOLYTICUM COLG COLLAGENASE COLLAGEN-BINDING DOMAIN 3B AT 1.65 ANGSTROM RESOLUTION IN PRESENCE OF CALCIUM

OverviewOverview

The crystal structure of a collagen-binding domain (CBD) with an N-terminal domain linker from Clostridium histolyticum class I collagenase was determined at 1.00 A resolution in the absence of calcium (1NQJ) and at 1.65 A resolution in the presence of calcium (1NQD). The mature enzyme is composed of four domains: a metalloprotease domain, a spacing domain and two CBDs. A 12-residue-long linker is found at the N-terminus of each CBD. In the absence of calcium, the CBD reveals a beta-sheet sandwich fold with the linker adopting an alpha-helix. The addition of calcium unwinds the linker and anchors it to the distal side of the sandwich as a new beta-strand. The conformational change of the linker upon calcium binding is confirmed by changes in the Stokes and hydrodynamic radii as measured by size exclusion chromatography and by dynamic light scattering with and without calcium. Furthermore, extensive mutagenesis of conserved surface residues and collagen-binding studies allow us to identify the collagen-binding surface of the protein and propose likely collagen-protein binding models.

About this StructureAbout this Structure

1NQD is a Single protein structure of sequence from Clostridium histolyticum. Full crystallographic information is available from OCA.

ReferenceReference

A bacterial collagen-binding domain with novel calcium-binding motif controls domain orientation., Wilson JJ, Matsushita O, Okabe A, Sakon J, EMBO J. 2003 Apr 15;22(8):1743-52. PMID:12682007 Page seeded by OCA on Sat May 3 02:51:17 2008

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OCA

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 [[Image:1nqd.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1nqd |SIZE=350|CAPTION= <scene name='initialview01'>1nqd</scene>, resolution 1.65&Aring;
+The line below this paragraph, containing "STRUCTURE_1nqd", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Microbial_collagenase Microbial collagenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.3 3.4.24.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= ColG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1498 Clostridium histolyticum])
+-->
-|DOMAIN=
+{{STRUCTURE_1nqd|  PDB=1nqd  |  SCENE=  }}
-|RELATEDENTRY=[[1nqj|1NQJ]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nqd OCA], [http://www.ebi.ac.uk/pdbsum/1nqd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nqd RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF CLOSTRIDIUM HISTOLYTICUM COLG COLLAGENASE COLLAGEN-BINDING DOMAIN 3B AT 1.65 ANGSTROM RESOLUTION IN PRESENCE OF CALCIUM'''
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 [[Category: Sakon, J.]]
 [[Category: Wilson, J J.]]
-[[Category: beta sandwich]]
+[[Category: Beta sandwich]]
-[[Category: calcium]]
+[[Category: Calcium]]
-[[Category: collagen-binding domain]]
+[[Category: Collagen-binding domain]]
-[[Category: metalloprotease]]
+[[Category: Metalloprotease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:51:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:34:03 2008''