1noc: Difference between revisions

Revision as of 02:46, 3 May 2008

MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114) COMPLEXED WITH TYPE I E. COLI CHLORAMPHENICOL ACETYL TRANSFERASE AND IMIDAZOLE

OverviewOverview

The nitric oxide synthase oxygenase domain (NOSox) oxidizes arginine to synthesize the cellular signal and defensive cytotoxin nitric oxide (NO). Crystal structures determined for cytokine-inducible NOSox reveal an unusual fold and heme environment for stabilization of activated oxygen intermediates key for catalysis. A winged beta sheet engenders a curved alpha-beta domain resembling a baseball catcher's mitt with heme clasped in the palm. The location of exposed hydrophobic residues and the results of mutational analysis place the dimer interface adjacent to the heme-binding pocket. Juxtaposed hydrophobic O2- and polar L-arginine-binding sites occupied by imidazole and aminoguanidine, respectively, provide a template for designing dual-function inhibitors and imply substrate-assisted catalysis.

About this StructureAbout this Structure

1NOC is a Protein complex structure of sequences from Escherichia coli and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

The structure of nitric oxide synthase oxygenase domain and inhibitor complexes., Crane BR, Arvai AS, Gachhui R, Wu C, Ghosh DK, Getzoff ED, Stuehr DJ, Tainer JA, Science. 1997 Oct 17;278(5337):425-31. PMID:9334294 Page seeded by OCA on Sat May 3 02:46:41 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1noc.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1noc |SIZE=350|CAPTION= <scene name='initialview01'>1noc</scene>, resolution 2.6&Aring;
+The line below this paragraph, containing "STRUCTURE_1noc", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1noc|  PDB=1noc  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1noc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1noc OCA], [http://www.ebi.ac.uk/pdbsum/1noc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1noc RCSB]</span>
-}}
 '''MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DOMAIN (DELTA 114) COMPLEXED WITH TYPE I E. COLI CHLORAMPHENICOL ACETYL TRANSFERASE AND IMIDAZOLE'''
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 [[Category: Stuehr, D J.]]
 [[Category: Tainer, J A.]]
-[[Category: cat]]
+[[Category: Cat]]
-[[Category: complex (oxidoreductase/transferase)]]
+[[Category: Heme]]
-[[Category: heme]]
+[[Category: Imidazole]]
-[[Category: imidazole]]
+[[Category: L-arginine monooxygenase]]
-[[Category: l-arginine monooxygenase]]
+[[Category: Nitric oxide]]
-[[Category: nitric oxide]]
+[[Category: No]]
-[[Category: no]]
+[[Category: No]]
-[[Category: no]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:46:41 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:33:09 2008''