5lv1: Difference between revisions
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==2.12 A resolution structure of PtxB from Prochlorococcus marinus (MIT 9301) in complex with phosphite== | ==2.12 A resolution structure of PtxB from Prochlorococcus marinus (MIT 9301) in complex with phosphite== | ||
<StructureSection load='5lv1' size='340' side='right' caption='[[5lv1]], [[Resolution|resolution]] 2.12Å' scene=''> | <StructureSection load='5lv1' size='340' side='right'caption='[[5lv1]], [[Resolution|resolution]] 2.12Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5lv1]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5lv1]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Prochlorococcus_marinus_str._MIT_9301 Prochlorococcus marinus str. MIT 9301]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LV1 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.12Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78T:oxidanylphosphinate'>78T</scene></td></tr> | |||
<tr id=' | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lv1 OCA], [https://pdbe.org/5lv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lv1 RCSB], [https://www.ebi.ac.uk/pdbsum/5lv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lv1 ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/PHND2_PROM0 PHND2_PROM0] Probably part of the ABC transporter complex PhnC2D2E2. Binds strongly to methylphosphonate (MPn), ethylphosphonate (EPn) and inorganic phosphite.<ref>PMID:22011717</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Adams | [[Category: Prochlorococcus marinus str. MIT 9301]] | ||
[[Category: Bibby | [[Category: Adams NBP]] | ||
[[Category: Bisson | [[Category: Bibby TS]] | ||
[[Category: Hitchcock | [[Category: Bisson C]] | ||
[[Category: Hunter | [[Category: Hitchcock A]] | ||
[[Category: Polyviou | [[Category: Hunter CN]] | ||
[[Category: Polyviou D]] | |||
Latest revision as of 21:50, 18 October 2023
2.12 A resolution structure of PtxB from Prochlorococcus marinus (MIT 9301) in complex with phosphite2.12 A resolution structure of PtxB from Prochlorococcus marinus (MIT 9301) in complex with phosphite
Structural highlights
FunctionPHND2_PROM0 Probably part of the ABC transporter complex PhnC2D2E2. Binds strongly to methylphosphonate (MPn), ethylphosphonate (EPn) and inorganic phosphite.[1] Publication Abstract from PubMedInorganic phosphate is the major bioavailable form of the essential nutrient phosphorus. However, the concentration of phosphate in most natural habitats is low enough to limit microbial growth. Under phosphate-depleted conditions some bacteria utilise phosphite and hypophosphite as alternative sources of phosphorus, but the molecular basis of reduced phosphorus acquisition from the environment is not fully understood. Here, we present crystal structures and ligand binding affinities of periplasmic binding proteins from bacterial phosphite and hypophosphite ATP-binding cassette transporters. We reveal that phosphite and hypophosphite specificity results from a combination of steric selection and the presence of a P-H...pi interaction between the ligand and a conserved aromatic residue in the ligand-binding pocket. The characterisation of high affinity and specific transporters has implications for the marine phosphorus redox cycle, and might aid the use of phosphite as an alternative phosphorus source in biotechnological, industrial and agricultural applications. The molecular basis of phosphite and hypophosphite recognition by ABC-transporters.,Bisson C, Adams NBP, Stevenson B, Brindley AA, Polyviou D, Bibby TS, Baker PJ, Hunter CN, Hitchcock A Nat Commun. 2017 Nov 23;8(1):1746. doi: 10.1038/s41467-017-01226-8. PMID:29170493[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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