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<SX load='2ykr' size='340' side='right' viewer='molstar' caption='[[2ykr]], [[Resolution|resolution]] 9.80&Aring;' scene=''>
<SX load='2ykr' size='340' side='right' viewer='molstar' caption='[[2ykr]], [[Resolution|resolution]] 9.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ykr]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_dh5[alpha] Escherichia coli dh5[alpha]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YKR FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ykr]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_DH5alpha Escherichia coli DH5alpha]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YKR FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2vhp|2vhp]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ykr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ykr OCA], [https://pdbe.org/2ykr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ykr RCSB], [https://www.ebi.ac.uk/pdbsum/2ykr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ykr ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ykr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ykr OCA], [https://pdbe.org/2ykr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ykr RCSB], [https://www.ebi.ac.uk/pdbsum/2ykr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ykr ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/RS11_ECO8A RS11_ECO8A]] Located on the platform of the 30S subunit, it bridges several disparate RNA helices of the 16S rRNA. Forms part of the Shine-Dalgarno cleft in the 70S ribosome. [[https://www.uniprot.org/uniprot/E3XT25_ECOLX E3XT25_ECOLX]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, probably blocks exit of the E-site tRNA.[HAMAP-Rule:MF_00480] [[https://www.uniprot.org/uniprot/RS17_ECO57 RS17_ECO57]] One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA (By similarity). [[https://www.uniprot.org/uniprot/E3PE32_ECOH1 E3PE32_ECOH1]] May play a role in 30S ribosomal subunit biogenesis. Unusual circulary permuted GTPase that catalyzes rapid hydrolysis of GTP with a slow catalytic turnover.[HAMAP-Rule:MF_01820][SAAS:SAAS00087566] [[https://www.uniprot.org/uniprot/E3PE70_ECOH1 E3PE70_ECOH1]] Binds as a heterodimer with protein S6 to the central domain of the 16S rRNA, where it helps stabilize the platform of the 30S subunit.[HAMAP-Rule:MF_00270] [[https://www.uniprot.org/uniprot/RS13_ECOK1 RS13_ECOK1]] Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement. Contacts the tRNAs in the A and P-sites. [[https://www.uniprot.org/uniprot/RS6_ECOSE RS6_ECOSE]] Binds together with S18 to 16S ribosomal RNA. [[https://www.uniprot.org/uniprot/RS3_ECOK1 RS3_ECOK1]] Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation. [[https://www.uniprot.org/uniprot/RS12_ECOL6 RS12_ECOL6]] With S4 and S5 plays an important role in translational accuracy.  Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity). [[https://www.uniprot.org/uniprot/RS14_ECO8A RS14_ECO8A]] Binds 16S rRNA, required for the assembly of 30S particles and may also be responsible for determining the conformation of the 16S rRNA at the A site. [[https://www.uniprot.org/uniprot/B3HGB0_ECOLX B3HGB0_ECOLX]] Forms an intersubunit bridge (bridge B4) with the 23S rRNA of the 50S subunit in the ribosome.[HAMAP-Rule:MF_01343]  One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it helps nucleate assembly of the platform of the 30S subunit by binding and bridging several RNA helices of the 16S rRNA.[HAMAP-Rule:MF_01343][RuleBase:RU004524] [[https://www.uniprot.org/uniprot/RS20_ECO55 RS20_ECO55]] Binds directly to 16S ribosomal RNA. [[https://www.uniprot.org/uniprot/RS8_ECOSE RS8_ECOSE]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit. [[https://www.uniprot.org/uniprot/RS4_ECOK1 RS4_ECOK1]] One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit (By similarity).  With S5 and S12 plays an important role in translational accuracy (By similarity). [[https://www.uniprot.org/uniprot/RS10_ECO24 RS10_ECO24]] Involved in the binding of tRNA to the ribosomes. [[https://www.uniprot.org/uniprot/RS19_ECOSE RS19_ECOSE]] Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA. [[https://www.uniprot.org/uniprot/D6JG86_ECOLX D6JG86_ECOLX]] Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.[HAMAP-Rule:MF_01307][SAAS:SAAS00085417]  With S4 and S12 plays an important role in translational accuracy.[HAMAP-Rule:MF_01307][SAAS:SAAS00085429]  
[https://www.uniprot.org/uniprot/RS2_ECOLI RS2_ECOLI]  
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== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
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[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chen, K]]
[[Category: Chen K]]
[[Category: Feng, B]]
[[Category: Feng B]]
[[Category: Gao, N]]
[[Category: Gao N]]
[[Category: Guo, Q]]
[[Category: Guo Q]]
[[Category: Lei, J]]
[[Category: Lei J]]
[[Category: Liu, L]]
[[Category: Liu L]]
[[Category: Xu, Y]]
[[Category: Xu Y]]
[[Category: Yuan, Y]]
[[Category: Yuan Y]]
[[Category: Circularly permutated gtpase]]
[[Category: Ribosome biogenesis]]
[[Category: Ribosome-hydrolase complex]]
[[Category: Yjeq]]

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