7st8: Difference between revisions
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==Crystal structure of 7H2.2 Fab in complex with SAS1B C-terminal region== | ==Crystal structure of 7H2.2 Fab in complex with SAS1B C-terminal region== | ||
<StructureSection load='7st8' size='340' side='right'caption='[[7st8]]' scene=''> | <StructureSection load='7st8' size='340' side='right'caption='[[7st8]], [[Resolution|resolution]] 2.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ST8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ST8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[7st8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ST8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ST8 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7st8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7st8 OCA], [https://pdbe.org/7st8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7st8 RCSB], [https://www.ebi.ac.uk/pdbsum/7st8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7st8 ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7st8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7st8 OCA], [https://pdbe.org/7st8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7st8 RCSB], [https://www.ebi.ac.uk/pdbsum/7st8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7st8 ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Disease == | |||
[https://www.uniprot.org/uniprot/ASTL_HUMAN ASTL_HUMAN] The disease may be caused by variants affecting the gene represented in this entry. | |||
== Function == | |||
[https://www.uniprot.org/uniprot/ASTL_HUMAN ASTL_HUMAN] Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.[UniProtKB:Q6HA09] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure of the antigen-binding fragment (Fab) of mouse monoclonal antibody 7H2.2 in complex with a 15-residue fragment from the metalloproteinase sperm acrosomal SLLP1 binding protein (SAS1B), which is a molecular and cellular candidate for both cancer therapy and female contraception, has been determined at 2.75 A resolution by single-crystal X-ray diffraction. Although the crystallization conditions contained the final 148 C-terminal residues of SAS1B, the Fab was observed to crystallize in complex with a 15-residue fragment corresponding to one of only two elements of secondary structure that are predicted to be ordered within the C-terminal region of SAS1B. The antigen forms an amphipathic alpha-helix that binds the 7H2.2 combining site via hydrophilic residues in an epitope that spans the length of the antigen alpha-helix, with only two CH-pi interactions observed along the edge of the interface between the antibody and antigen. Interestingly, the paratope contains two residues mutated away from the germline (YL32F and YH58R), as well as a ProH96-ThrH97-AspH98-AspH99 insertion within heavy chain CDR3. The intact 7H2.2 antibody exhibits high affinity for the SAS1B antigen, with 1:1 binding and nanomolar affinity for both the SAS1B C-terminal construct used for crystallization (3.38 +/- 0.59 nM) and a 15-amino-acid synthetic peptide construct corresponding to the helical antigen observed within the crystal structure (1.60 +/- 0.31 nM). The SAS1B-antibody structure provides the first structural insight into any portion of the subdomain architecture of the C-terminal region of the novel cancer-oocyte tumor surface neoantigen SAS1B and provides a basis for the targeted use of SAS1B. | |||
Monoclonal antibody 7H2.2 binds the C-terminus of the cancer-oocyte antigen SAS1B through the hydrophilic face of a conserved amphipathic helix corresponding to one of only two regions predicted to be ordered.,Legg MSG, Gagnon SML, Powell CJ, Boulanger MJ, Li AJJ, Evans SV Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):623-632. doi: , 10.1107/S2059798322003011. Epub 2022 Apr 20. PMID:35503210<ref>PMID:35503210</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 7st8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Antibody 3D structures|Antibody 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Mus musculus]] | |||
[[Category: Evans SV]] | [[Category: Evans SV]] | ||
[[Category: Legg MSG]] | [[Category: Legg MSG]] | ||