1nld: Difference between revisions
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'''FAB FRAGMENT OF A NEUTRALIZING ANTIBODY DIRECTED AGAINST AN EPITOPE OF GP41 FROM HIV-1''' | '''FAB FRAGMENT OF A NEUTRALIZING ANTIBODY DIRECTED AGAINST AN EPITOPE OF GP41 FROM HIV-1''' | ||
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[[Category: Muirhead, H.]] | [[Category: Muirhead, H.]] | ||
[[Category: Rawas, A.]] | [[Category: Rawas, A.]] | ||
[[Category: | [[Category: Fab fragment]] | ||
[[Category: | [[Category: Immunoglobulin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:40:13 2008'' | |||
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Revision as of 02:40, 3 May 2008
FAB FRAGMENT OF A NEUTRALIZING ANTIBODY DIRECTED AGAINST AN EPITOPE OF GP41 FROM HIV-1
OverviewOverview
The structure of a Fab fragment of a monoclonal antibody (1583) that neutralizes a broad range of HIV-1 isolates has been solved by X-ray crystallography. This antibody is directed against a poliovirus/HIV-I chimaera which presents a conserved epitope of the envelope protein gp41. Crystals of 1583 were obtained in the space group P2(1)2(1)2(1) and the structure solved by molecular replacement. The model has been refined against all data in the range 10-2.9 A to a final crystallographic R factor of 0.198. The antigen-binding site features a well defined groove, typical of antibodies that bind to small antigens, created in part by a relatively short CDR H3. The variable regions of 1583 were sequenced and, given the hydrophilic nature of the epitope, revealed a surprising lack of charged residues in the CDR's. However, the antigen-binding cleft is indeed very polar, due in part to the presence of two charged residues that emanate from outside the recognized CDR's.
About this StructureAbout this Structure
1NLD is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the Fab fragment from a neutralizing monoclonal antibody directed against an epitope of gp41 from HIV-1., Davies C, Beauchamp JC, Emery D, Rawas A, Muirhead H, Acta Crystallogr D Biol Crystallogr. 1997 Mar 1;53(Pt 2):186-94. PMID:15299953 Page seeded by OCA on Sat May 3 02:40:13 2008