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'''ADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE''' | '''ADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NK0 OCA]. | |||
==Reference== | ==Reference== | ||
Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15035983 15035983] | Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15035983 15035983] | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Beese, L S.]] | [[Category: Beese, L S.]] | ||
[[Category: Johnson, S J.]] | [[Category: Johnson, S J.]] | ||
[[Category: | [[Category: Dna mismatch]] | ||
[[Category: | [[Category: Dna polymerase i]] | ||
[[Category: | [[Category: Dna replication]] | ||
[[Category: | [[Category: Klenow fragment]] | ||
[[Category: | [[Category: Protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:37:36 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 02:37, 3 May 2008
ADENINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE
OverviewOverview
Accurate DNA replication is essential for genomic stability. One mechanism by which high-fidelity DNA polymerases maintain replication accuracy involves stalling of the polymerase in response to covalent incorporation of mismatched base pairs, thereby favoring subsequent mismatch excision. Some polymerases retain a "short-term memory" of replication errors, responding to mismatches up to four base pairs in from the primer terminus. Here we a present a structural characterization of all 12 possible mismatches captured at the growing primer terminus in the active site of a polymerase. Our observations suggest four mechanisms that lead to mismatch-induced stalling of the polymerase. Furthermore, we have observed the effects of extending a mismatch up to six base pairs from the primer terminus and find that long-range distortions in the DNA transmit the presence of the mismatch back to the enzyme active site, suggesting the structural basis for the short-term memory of replication errors.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:15035983 Page seeded by OCA on Sat May 3 02:37:36 2008