1njm: Difference between revisions

Revision as of 02:36, 3 May 2008

The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a tRNA acceptor stem mimic (ASM) and the antibiotic sparsomycin

OverviewOverview

Crystal structures of tRNA mimics complexed with the large ribosomal subunit of Deinococcus radiodurans indicate that remote interactions determine the precise orientation of tRNA in the peptidyl-transferase center (PTC). The PTC tolerates various orientations of puromycin derivatives and its flexibility allows the conformational rearrangements required for peptide-bond formation. Sparsomycin binds to A2602 and alters the PTC conformation. H69, the intersubunit-bridge connecting the PTC and decoding site, may also participate in tRNA placement and translocation. A spiral rotation of the 3' end of the A-site tRNA around a 2-fold axis of symmetry identified within the PTC suggests a unified ribosomal machinery for peptide-bond formation, A-to-P-site translocation, and entrance of nascent proteins into the exit tunnel. Similar 2-fold related regions, detected in all known structures of large ribosomal subunits, indicate the universality of this mechanism.

About this StructureAbout this Structure

1NJM is a Protein complex structure of sequences from Deinococcus radiodurans. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis of the ribosomal machinery for peptide bond formation, translocation, and nascent chain progression., Bashan A, Agmon I, Zarivach R, Schluenzen F, Harms J, Berisio R, Bartels H, Franceschi F, Auerbach T, Hansen HA, Kossoy E, Kessler M, Yonath A, Mol Cell. 2003 Jan;11(1):91-102. PMID:12535524 Page seeded by OCA on Sat May 3 02:36:37 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1njm.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1njm", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=PPU:PUROMYCIN-5&#39;-MONOPHOSPHATE'>PPU</scene>, <scene name='pdbligand=SPS:SPARSOMYCIN'>SPS</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1njn|1NJN]], [[1njo|1NJO]], [[1njp|1NJP]], [[1k01|1K01]], [[1jzx|1JZX]], [[1jzy|1JZY]], [[1jzz|1JZZ]], [[1k00|1K00]], [[1nkw|1NKW]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1njm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1njm OCA], [http://www.ebi.ac.uk/pdbsum/1njm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1njm RCSB]</span>
-}}
 '''The crystal structure of the 50S Large ribosomal subunit from Deinococcus radiodurans complexed with a tRNA acceptor stem mimic (ASM) and the antibiotic sparsomycin'''
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 [[Category: Yonath, A.]]
 [[Category: Zarivatch, R.]]
-[[Category: peptide bond formation]]
+[[Category: Peptide bond formation]]
-[[Category: peptidyl-transferase]]
+[[Category: Peptidyl-transferase]]
-[[Category: puromycin]]
+[[Category: Puromycin]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
-[[Category: sparsomycin]]
+[[Category: Sparsomycin]]
-[[Category: trna]]
+[[Category: Trna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:36:37 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:31:18 2008''