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'''Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit''' | '''Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit''' | ||
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[[Category: Podobnik, M.]] | [[Category: Podobnik, M.]] | ||
[[Category: Weitze, T F.]] | [[Category: Weitze, T F.]] | ||
[[Category: | [[Category: Aaa+ atpase domain]] | ||
[[Category: | [[Category: Rossman-like fold]] | ||
[[Category: | [[Category: Sensor 1]] | ||
[[Category: | [[Category: Sensor 2]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:36:21 2008'' | |||
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Revision as of 02:36, 3 May 2008
Nucleotide-free form of an Isolated E. coli Clamp Loader Gamma Subunit
OverviewOverview
Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.
About this StructureAbout this Structure
1NJG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:12623013 Page seeded by OCA on Sat May 3 02:36:21 2008