6wpl: Difference between revisions
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==Structure of Cytochrome P450tcu== | ==Structure of Cytochrome P450tcu== | ||
<StructureSection load='6wpl' size='340' side='right'caption='[[6wpl]]' scene=''> | <StructureSection load='6wpl' size='340' side='right'caption='[[6wpl]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPL OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6wpl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._TCU-HL1 Pseudomonas sp. TCU-HL1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WPL FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.097Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAH:5-EXO-HYDROXYCAMPHOR'>CAH</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wpl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wpl OCA], [https://pdbe.org/6wpl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wpl RCSB], [https://www.ebi.ac.uk/pdbsum/6wpl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wpl ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A1B3EAY9_9PSED A0A1B3EAY9_9PSED] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The bacterial cytochrome P450cam catalyzes the oxidation of camphor to 5-exo-hydroxycamphor as the first step in the oxidative assimilation of camphor as a carbon/energy source. CYP101D1 is another bacterial P450 that catalyzes the same reaction. A third P450 (P450tcu) has recently been discovered that has approximately 86% sequence identity to P450cam as well as very similar enzymatic properties. P450tcu, however, exhibits three unusual features not found in P450cam. First, we observe product in at least two orientations in the x-ray structure which indicates that, unlike P450cam, x-ray generated reducing equivalents can drive substrate hydroxylation in crystallo. We postulate, based on molecular dynamic simulations, that greater flexibility in P450tcu enables easier access of protons to the active site and, together with x-ray driven reduction, results in O2 activation and substrate hydroxylation. Second, the characteristic low- to -high-spin transition when camphor binds occurs immediately with P450cam but is very slow in P450tcu. Third, isothermal titration calorimetry shows that in P450cam substrate binding is entropically driven with DeltaH>0 while in P450tcu DeltaH<0 with a more modest change in -TDeltaS. These results indicate that despite nearly identical structures and enzymatic properties, these two P450s exhibit quite different properties most likely related to differences in conformational dynamics. | |||
Unexpected Differences Between Two Closely Related Bacterial P450 Camphor Monooxygenases.,Murarka VC, Batabyal D, Amaya JA, Sevrioukova I, Poulos TL Biochemistry. 2020 Jun 17. doi: 10.1021/acs.biochem.0c00366. PMID:32551522<ref>PMID:32551522</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6wpl" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Pseudomonas sp. TCU-HL1]] | |||
[[Category: Amaya JA]] | [[Category: Amaya JA]] | ||
[[Category: Batabyal D]] | [[Category: Batabyal D]] | ||