1nh8: Difference between revisions

Revision as of 02:31, 3 May 2008

ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH AMP AND HISTIDINE

OverviewOverview

The N-1-(5'-phosphoribosyl)-ATP transferase catalyzes the first step of the histidine biosynthetic pathway and is regulated by a feedback mechanism by the product histidine. The crystal structures of the N-1-(5'-phosphoribosyl)-ATP transferase from Mycobacterium tuberculosis in complex with inhibitor histidine and AMP has been determined to 1.8 A resolution and without ligands to 2.7 A resolution. The active enzyme exists primarily as a dimer, and the histidine-inhibited form is a hexamer. The structure represents a new fold for a phosphoribosyltransferase, consisting of three continuous domains. The inhibitor AMP binds in the active site cavity formed between the two catalytic domains. A model for the mechanism of allosteric inhibition has been derived from conformational differences between the AMP:His-bound and apo structures.

About this StructureAbout this Structure

1NH8 is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of ATP phosphoribosyltransferase from Mycobacterium tuberculosis., Cho Y, Sharma V, Sacchettini JC, J Biol Chem. 2003 Mar 7;278(10):8333-9. Epub 2003 Jan 2. PMID:12511575 Page seeded by OCA on Sat May 3 02:31:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1nh8.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1nh8 |SIZE=350|CAPTION= <scene name='initialview01'>1nh8</scene>, resolution 1.80&Aring;
+The line below this paragraph, containing "STRUCTURE_1nh8", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=HIS:HISTIDINE'>HIS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/ATP_phosphoribosyltransferase ATP phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.17 2.4.2.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam08029 HisG_C], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK00489 hisG], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam01634 HisG]</span>
+{{STRUCTURE_1nh8|  PDB=1nh8  |  SCENE=  }}
-|RELATEDENTRY=[[1nh7|1NH7]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nh8 OCA], [http://www.ebi.ac.uk/pdbsum/1nh8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nh8 RCSB]</span>
-}}
 '''ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH AMP AND HISTIDINE'''
@@ Line 30: / Line 27: @@
 [[Category: Sharma, V.]]
 [[Category: TBSGC, TB Structural Genomics Consortium.]]
-[[Category: atp]]
+[[Category: Atp]]
-[[Category: de novo his biosynthesis]]
+[[Category: De novo his biosynthesis]]
-[[Category: phosphoribosyltransferase]]
+[[Category: Phosphoribosyltransferase]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: prpp]]
+[[Category: Prpp]]
-[[Category: prtase]]
+[[Category: Prtase]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: tb structural genomics consortium]]
+[[Category: Tb structural genomics consortium]]
-[[Category: tbsgc]]
+[[Category: Tbsgc]]
-[[Category: transferase]]
+[[Category: Transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:31:36 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:30:20 2008''