6n2v: Difference between revisions
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<StructureSection load='6n2v' size='340' side='right'caption='[[6n2v]], [[Resolution|resolution]] 2.85Å' scene=''> | <StructureSection load='6n2v' size='340' side='right'caption='[[6n2v]], [[Resolution|resolution]] 2.85Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6n2v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N2V OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6n2v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_oryzae Xanthomonas oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6N2V FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6n2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n2v OCA], [https://pdbe.org/6n2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6n2v RCSB], [https://www.ebi.ac.uk/pdbsum/6n2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6n2v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 6n2v" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6n2v" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Riboswitch 3D structures|Riboswitch 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Xanthomonas oryzae]] | ||
[[Category: | [[Category: Ke A]] | ||
[[Category: | [[Category: Price IR]] | ||
Latest revision as of 09:44, 11 October 2023
Manganese riboswitch from Xanthmonas oryzae bound to Mn(II)Manganese riboswitch from Xanthmonas oryzae bound to Mn(II)
Structural highlights
Publication Abstract from PubMedThe widespread Mn(2+)-sensing yybP-ykoY riboswitch controls the expression of bacterial Mn(2+) homeostasis genes. Here, we first determine the crystal structure of the ligand-bound yybP-ykoY riboswitch aptamer from Xanthomonas oryzae at 2.96 A resolution, revealing two conformations with docked four-way junction (4WJ) and incompletely coordinated metal ions. In >100 micros of MD simulations, we observe that loss of divalents from the core triggers local structural perturbations in the adjacent docking interface, laying the foundation for signal transduction to the regulatory switch helix. Using single-molecule FRET, we unveil a previously unobserved extended 4WJ conformation that samples transient docked states in the presence of Mg(2+). Only upon adding sub-millimolar Mn(2+), however, can the 4WJ dock stably, a feature lost upon mutation of an adenosine contacting Mn(2+) in the core. These observations illuminate how subtly differing ligand preferences of competing metal ions become amplified by the coupling of local with global RNA dynamics. Local-to-global signal transduction at the core of a Mn(2+) sensing riboswitch.,Suddala KC, Price IR, Dandpat SS, Janecek M, Kuhrova P, Sponer J, Banas P, Ke A, Walter NG Nat Commun. 2019 Sep 20;10(1):4304. doi: 10.1038/s41467-019-12230-5. PMID:31541094[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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