1n8v: Difference between revisions
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'''Chemosensory Protein in complex with bromo-dodecanol''' | '''Chemosensory Protein in complex with bromo-dodecanol''' | ||
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[[Category: Lartigue, A.]] | [[Category: Lartigue, A.]] | ||
[[Category: Tegoni, M.]] | [[Category: Tegoni, M.]] | ||
[[Category: | [[Category: Lipid binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:14:17 2008'' | |||
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Revision as of 02:14, 3 May 2008
Chemosensory Protein in complex with bromo-dodecanol
OverviewOverview
Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of insect species. The x-ray structure of CSPMbraA6, a 112-aa antennal protein from the moth Mamestra brassicae (Mbra), was shown to exhibit a novel type of alpha-helical fold. We have performed a structural and binding study of CSPMbraA6 to get some insights into its possible molecular function. Tryptophan fluorescence quenching demonstrates the ability of CSPMbraA6 to bind several types of semio-chemicals or surrogate ligands with microM K(d). Its crystal structure in complex with one of these compounds, 12-bromo-dodecanol, reveals extensive conformational changes on binding, resulting in the formation of a large cavity filled by three ligand molecules. Furthermore, binding cooperativity was demonstrated for some ligands, suggesting a stepwise binding. The peculiar rearrangement of CSPMbraA6 conformation and the cooperativity phenomenon might trigger the recognition of chemicals by receptors and induce subsequent signal transduction.
About this StructureAbout this Structure
1N8V is a Single protein structure of sequence from Mamestra brassicae. Full crystallographic information is available from OCA.
ReferenceReference
Moth chemosensory protein exhibits drastic conformational changes and cooperativity on ligand binding., Campanacci V, Lartigue A, Hallberg BM, Jones TA, Giudici-Orticoni MT, Tegoni M, Cambillau C, Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5069-74. Epub 2003 Apr 15. PMID:12697900 Page seeded by OCA on Sat May 3 02:14:17 2008