1n5y: Difference between revisions

Revision as of 02:08, 3 May 2008

HIV-1 Reverse Transcriptase Crosslinked to Post-Translocation AZTMP-Terminated DNA (Complex P)

OverviewOverview

AZT (3'-azido-3'-deoxythymidine) resistance involves the enhanced excision of AZTMP from the end of the primer strand by HIV-1 reverse transcriptase. This reaction can occur when an AZTMP-terminated primer is bound at the nucleotide-binding site (pre-translocation complex N) but not at the 'priming' site (post-translocation complex P). We determined the crystal structures of N and P complexes at 3.0 and 3.1 A resolution. These structures provide insight into the structural basis of AZTMP excision and the mechanism of translocation. Docking of a dNTP in the P complex structure suggests steric crowding in forming a stable ternary complex that should increase the relative amount of the N complex, which is the substrate for excision. Structural differences between complexes N and P suggest that the conserved YMDD loop is involved in translocation, acting as a springboard that helps to propel the primer terminus from the N to the P site after dNMP incorporation.

About this StructureAbout this Structure

1N5Y is a Protein complex structure of sequences from Human immunodeficiency virus 1 and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structures of HIV-1 reverse transcriptase with pre- and post-translocation AZTMP-terminated DNA., Sarafianos SG, Clark AD Jr, Das K, Tuske S, Birktoft JJ, Ilankumaran P, Ramesha AR, Sayer JM, Jerina DM, Boyer PL, Hughes SH, Arnold E, EMBO J. 2002 Dec 2;21(23):6614-24. PMID:12456667 Page seeded by OCA on Sat May 3 02:08:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1n5y.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1n5y |SIZE=350|CAPTION= <scene name='initialview01'>1n5y</scene>, resolution 3.10&Aring;
+The line below this paragraph, containing "STRUCTURE_1n5y", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ATM:3&#39;-AZIDO-3&#39;-DEOXYTHYMIDINE-5&#39;-MONOPHOSPHATE'>ATM</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MRG:N2-(3-MERCAPTOPROPYL)-2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>MRG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])
+-->
-|DOMAIN=
+{{STRUCTURE_1n5y|  PDB=1n5y  |  SCENE=  }}
-|RELATEDENTRY=[[1hys|1HYS]], [[2hmi|2HMI]], [[1rtd|1RTD]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5y OCA], [http://www.ebi.ac.uk/pdbsum/1n5y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n5y RCSB]</span>
-}}
 '''HIV-1 Reverse Transcriptase Crosslinked to Post-Translocation AZTMP-Terminated DNA (Complex P)'''
@@ Line 39: / Line 36: @@
 [[Category: Sayer, J M.]]
 [[Category: Tuske, S.]]
-[[Category: complex p]]
+[[Category: Complex p]]
-[[Category: drug resistance]]
+[[Category: Drug resistance]]
-[[Category: hiv]]
+[[Category: Hiv]]
-[[Category: nucleotide excision]]
+[[Category: Nucleotide excision]]
-[[Category: translocation]]
+[[Category: Translocation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:08:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:52 2008''