1n5u: Difference between revisions

Revision as of 02:07, 3 May 2008

X-RAY STUDY OF HUMAN SERUM ALBUMIN COMPLEXED WITH HEME

OverviewOverview

The high resolution structure of hemalbumin was determined by single crystal X-ray diffraction to a resolution of 1.9 A. The structure revealed the protoporphyrin IX bound to a single site within a hydrophobic cavity in subdomain IB, one of the principal binding sites for long chain fatty acid. The iron is penta coordinated with the fifth ligand comprised of the hydroxyl oxygen of Tyr-161 (phenolic oxygen to heme plane distance: 2.73 A) in an otherwise completely hydrophobic pocket. The heme propionic acid residues form salt bridges with His-142 and Lys-190, which together with a series of hydrophobic interactions, enclose and secure the heme within the IB helical motif. A detailed discussion of the structure together with its implications for the development of potential blood substitutes is presented.

About this StructureAbout this Structure

1N5U is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The atomic structure of human methemalbumin at 1.9 A., Wardell M, Wang Z, Ho JX, Robert J, Ruker F, Ruble J, Carter DC, Biochem Biophys Res Commun. 2002 Mar 8;291(4):813-9. PMID:11866438 Page seeded by OCA on Sat May 3 02:07:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1n5u.gif|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1n5u", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n5u OCA], [http://www.ebi.ac.uk/pdbsum/1n5u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n5u RCSB]</span>
-}}
 '''X-RAY STUDY OF HUMAN SERUM ALBUMIN COMPLEXED WITH HEME'''
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 [[Category: Wang, Z.]]
 [[Category: Wardell, M.]]
-[[Category: plasma protein]]
+[[Category: Plasma protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:07:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:50 2008''