1n4w: Difference between revisions

Revision as of 02:06, 3 May 2008

ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ pH 7.3 (STREPTOMYCES SP. SA-COO)

OverviewOverview

Hydrogen atoms are a vital component of enzyme structure and function. In recent years, atomic resolution crystallography (>or=1.2 A) has been successfully used to investigate the role of the hydrogen atom in enzymatic catalysis. Here, atomic resolution crystallography was used to study the effect of pH on cholesterol oxidase from Streptomyces sp., a flavoenzyme oxidoreductase. Crystallographic observations of the anionic oxidized flavin cofactor at basic pH are consistent with the UV-visible absorption profile of the enzyme and readily explain the reversible pH-dependent loss of oxidation activity. Furthermore, a hydrogen atom, positioned at an unusually short distance from the main chain carbonyl oxygen of Met122 at high pH, was observed, suggesting a previously unknown mechanism of cofactor stabilization. This study shows how a redox active site responds to changes in the enzyme's environment and how these changes are able to influence the mechanism of enzymatic catalysis.

About this StructureAbout this Structure

1N4W is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.

ReferenceReference

Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment., Lyubimov AY, Lario PI, Moustafa I, Vrielink A, Nat Chem Biol. 2006 May;2(5):259-64. Epub 2006 Apr 9. PMID:16604066 Page seeded by OCA on Sat May 3 02:06:06 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1n4w.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1n4w |SIZE=350|CAPTION= <scene name='initialview01'>1n4w</scene>, resolution 0.92&Aring;
+The line below this paragraph, containing "STRUCTURE_1n4w", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cholesterol_oxidase Cholesterol oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.6 1.1.3.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= CHOA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.])
+-->
-|DOMAIN=
+{{STRUCTURE_1n4w|  PDB=1n4w  |  SCENE=  }}
-|RELATEDENTRY=[[1b4v|1B4V]], [[1b8s|1B8S]], [[1cbo|1CBO]], [[1cc2|1CC2]], [[1ijh|1IJH]], [[1n1p|1N1P]], [[1n4v|1N4V]], [[1n4u|1N4U]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4w OCA], [http://www.ebi.ac.uk/pdbsum/1n4w PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n4w RCSB]</span>
-}}
 '''ATOMIC RESOLUTION STRUCTURE OF CHOLESTEROL OXIDASE @ pH 7.3 (STREPTOMYCES SP. SA-COO)'''
@@ Line 28: / Line 25: @@
 [[Category: Lario, P I.]]
 [[Category: Vrielink, A.]]
-[[Category: atomic resolution]]
+[[Category: Atomic resolution]]
-[[Category: flavoenzyme]]
+[[Category: Flavoenzyme]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
-[[Category: steroid metabolism]]
+[[Category: Steroid metabolism]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:06:06 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:31 2008''