1n4k: Difference between revisions

Revision as of 02:05, 3 May 2008

Crystal structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with IP3

OverviewOverview

In a variety of cells, the Ca2+ signalling process is mediated by the endoplasmic-reticulum-membrane-associated Ca2+ release channel, inositol 1,4,5-trisphosphate (InsP3) receptor (InsP3R). Being ubiquitous and present in organisms ranging from humans to Caenorhabditis elegans, InsP3R has a vital role in the control of cellular and physiological processes as diverse as cell division, cell proliferation, apoptosis, fertilization, development, behaviour, memory and learning. Mouse type I InsP3R (InsP3R1), found in high abundance in cerebellar Purkinje cells, is a polypeptide with three major functionally distinct regions: the amino-terminal InsP3-binding region, the central modulatory region and the carboxy-terminal channel region. Here we present a 2.2-A crystal structure of the InsP3-binding core of mouse InsP3R1 in complex with InsP3. The asymmetric, boomerang-like structure consists of an N-terminal beta-trefoil domain and a C-terminal alpha-helical domain containing an 'armadillo repeat'-like fold. The cleft formed by the two domains exposes a cluster of arginine and lysine residues that coordinate the three phosphoryl groups of InsP3. Putative Ca2+-binding sites are identified in two separate locations within the InsP3-binding core.

About this StructureAbout this Structure

1N4K is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with its ligand., Bosanac I, Alattia JR, Mal TK, Chan J, Talarico S, Tong FK, Tong KI, Yoshikawa F, Furuichi T, Iwai M, Michikawa T, Mikoshiba K, Ikura M, Nature. 2002 Dec 12;420(6916):696-700. Epub 2002 Nov 17. PMID:12442173 Page seeded by OCA on Sat May 3 02:05:13 2008

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OCA

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 [[Image:1n4k.jpg|left|200px]]
- {{Structure
+<!--
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+The line below this paragraph, containing "STRUCTURE_1n4k", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=I3P:D-MYO-INOSITOL-1,4,5-TRIPHOSPHATE'>I3P</scene>
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-|GENE= ITPR1 OR INSP3R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4k OCA], [http://www.ebi.ac.uk/pdbsum/1n4k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n4k RCSB]</span>
-}}
 '''Crystal structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with IP3'''
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 [[Category: Tong, K I.]]
 [[Category: Yoshikawa, F.]]
-[[Category: armadillo-like fold]]
+[[Category: Armadillo-like fold]]
-[[Category: b-trefoil fold]]
+[[Category: B-trefoil fold]]
-[[Category: calcium channel,protein-ligand complex]]
+[[Category: Calcium channel,protein-ligand complex]]
-[[Category: ip3 receptor]]
+[[Category: Ip3 receptor]]
-[[Category: ip3-binding core]]
+[[Category: Ip3-binding core]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:05:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:17 2008''