1n4f: Difference between revisions

Revision as of 02:05, 3 May 2008

Para-Arsanilate Derivative of Hen Egg-White Lysozyme

OverviewOverview

Single/multiple-wavelength anomalous dispersion (SAD/MAD) experiments were performed on a crystal of an organic arsenic derivative of hen egg-white lysozyme. A para-arsanilate compound used as a crystallizing reagent was incorporated into the ordered solvent region of the lysozyme molecule. Diffraction data were collected to high resolution (</=2.0 A) at three wavelengths around the K edge (1.04 A) of arsenic at beamline BM30A, ESRF synchrotron. Anomalous Patterson maps clearly showed the main arsanilate site to be between three symmetry-related lysozyme molecules, at a location previously occupied by a para-toluenesulfonate anion. MAD phases at 2 A derived using the program SHARP led to an electron-density map of sufficient quality to start manual building of the protein model. Amplitudes from a second crystal measured to a resolution of 1.8 A at the peak wavelength revealed two additional heavy-atom sites, which reinforced the anomalous subset model and therefore dramatically improved the phasing power of the arsenic derivative. The subsequent solvent-flattened map was of such high accuracy that the program ARP/wARP was able to build a nearly complete model automatically. This work emphasizes the great potential of arsenic for de novo structure determination using anomalous dispersion methods.

About this StructureAbout this Structure

1N4F is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Phasing power at the K absorption edge of organic arsenic., Retailleau P, Prange T, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):887-96. Epub 2003, Apr 25. PMID:12777806 Page seeded by OCA on Sat May 3 02:05:02 2008

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OCA

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 [[Image:1n4f.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1n4f", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ASR:4-AMINOPHENYLARSONIC+ACID'>ASR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
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-|RELATEDENTRY=[[1b0d|1B0D]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n4f OCA], [http://www.ebi.ac.uk/pdbsum/1n4f PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n4f RCSB]</span>
-}}
 '''Para-Arsanilate Derivative of Hen Egg-White Lysozyme'''
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 [[Category: Prange, T.]]
 [[Category: Retailleau, P.]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:05:02 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:25:14 2008''