5cz3: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5cz3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxoma_virus Myxoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CZ3 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5cz3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxoma_virus Myxoma virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CZ3 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cz3 OCA], [https://pdbe.org/5cz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cz3 RCSB], [https://www.ebi.ac.uk/pdbsum/5cz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cz3 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cz3 OCA], [https://pdbe.org/5cz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cz3 RCSB], [https://www.ebi.ac.uk/pdbsum/5cz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cz3 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
Latest revision as of 11:41, 27 September 2023
Crystal Structure of Myxoma Virus M64Crystal Structure of Myxoma Virus M64
Structural highlights
FunctionVH23_MYXVL Plays a role for multiplication of the virus in different cell types. Publication Abstract from PubMedHuman sterile alpha motif domain-containing 9 (SAMD9) protein is a host restriction factor for poxviruses, but it can be overcome by some poxvirus host-range proteins that share homology with vaccinia virus C7 protein. To understand the mechanism of action for this important family of host-range factors, we determined the crystal structures of C7 and myxoma virus M64, a C7 family member that is unable to antagonize SAMD9. Despite their different functions and only 23% sequence identity, the two proteins have very similar overall structures, displaying a previously unidentified fold comprised of a compact 12-stranded antiparallel beta-sandwich wrapped in two short alpha helices. Extensive structure-guided mutagenesis of C7 identified three loops clustered on one edge of the beta sandwich as critical for viral replication and binding with SAMD9. The loops are characterized with functionally important negatively charged, positively charged, and hydrophobic residues, respectively, together forming a unique "three-fingered molecular claw." The key residues of the claw are not conserved in two C7 family members that do not antagonize SAMD9 but are conserved in distantly related C7 family members from four poxvirus genera that infect diverse mammalian species. Indeed, we found that all in the latter group of proteins bind SAMD9. Taken together, our data indicate that diverse mammalian poxviruses use a conserved molecular claw in a C7-like protein to target SAMD9 and overcome host restriction. Structural basis for antagonizing a host restriction factor by C7 family of poxvirus host-range proteins.,Meng X, Krumm B, Li Y, Deng J, Xiang Y Proc Natl Acad Sci U S A. 2015 Nov 17. pii: 201515354. PMID:26578811[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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