4zsx: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4zsx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZSX FirstGlance]. <br> | <table><tr><td colspan='2'>[[4zsx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZSX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZSX FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zsx OCA], [https://pdbe.org/4zsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zsx RCSB], [https://www.ebi.ac.uk/pdbsum/4zsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zsx ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zsx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zsx OCA], [https://pdbe.org/4zsx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zsx RCSB], [https://www.ebi.ac.uk/pdbsum/4zsx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zsx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
Latest revision as of 11:23, 27 September 2023
Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-2.0Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-2.0
Structural highlights
FunctionPublication Abstract from PubMedConnecting proteins together in prescribed geometric arrangements is an important element in new areas of biomolecular design. In this study, we characterize the degree of three-dimensional orientational control that can be achieved when two protein domains that have alpha-helical termini are joined using an alpha-helical linker. A fusion between naturally oligomeric protein domains was designed in this fashion with the intent of creating a self-assembling 12-subunit tetrahedral protein cage. While the designed fusion protein failed to assemble into a tetrahedral cage in high yield, a series of crystal structures showed that the two fused components were indeed bridged by an intact alpha helix, although the fusion protein was distorted from the intended ideal configuration by bending of the helix, ranging from 7 to 35 degrees . That range of deviation in orientation creates challenges for designing large, perfectly symmetric protein assemblies, although it should offer useful outcomes for other less geometrically demanding applications in synthetic biology. On the predictability of the orientation of protein domains joined by a spanning alpha-helical linker.,Lai YT, Jiang L, Chen W, Yeates TO Protein Eng Des Sel. 2015 Aug 4. pii: gzv035. PMID:26243886[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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