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==Crystal structure of the light-driven sodium pump KR2 bound with iodide ions== | ==Crystal structure of the light-driven sodium pump KR2 bound with iodide ions== | ||
<StructureSection load='5jrf' size='340' side='right' caption='[[5jrf]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='5jrf' size='340' side='right'caption='[[5jrf]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5jrf]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[5jrf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dokdonia_eikasta Dokdonia eikasta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JRF FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jrf OCA], [https://pdbe.org/5jrf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jrf RCSB], [https://www.ebi.ac.uk/pdbsum/5jrf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jrf ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/N0DKS8_9FLAO N0DKS8_9FLAO] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5jrf" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5jrf" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Dokdonia eikasta]] | ||
[[Category: Gordeliy | [[Category: Large Structures]] | ||
[[Category: Gushchin | [[Category: Gordeliy V]] | ||
[[Category: Kovalev | [[Category: Gushchin I]] | ||
[[Category: Melnikov | [[Category: Kovalev K]] | ||
[[Category: Polovinkin | [[Category: Melnikov I]] | ||
[[Category: Popov | [[Category: Polovinkin V]] | ||
[[Category: Shevchenko | [[Category: Popov A]] | ||
[[Category: Shevchenko V]] | |||
Latest revision as of 22:03, 20 September 2023
Crystal structure of the light-driven sodium pump KR2 bound with iodide ionsCrystal structure of the light-driven sodium pump KR2 bound with iodide ions
Structural highlights
FunctionPublication Abstract from PubMedWe describe a fast, easy, and potentially universal method for the de novo solution of the crystal structures of membrane proteins via iodide-single-wavelength anomalous diffraction (I-SAD). The potential universality of the method is based on a common feature of membrane proteins-the availability at the hydrophobic-hydrophilic interface of positively charged amino acid residues with which iodide strongly interacts. We demonstrate the solution using I-SAD of four crystal structures representing different classes of membrane proteins, including a human G protein-coupled receptor (GPCR), and we show that I-SAD can be applied using data collection strategies based on either standard or serial x-ray crystallography techniques. Fast iodide-SAD phasing for high-throughput membrane protein structure determination.,Melnikov I, Polovinkin V, Kovalev K, Gushchin I, Shevtsov M, Shevchenko V, Mishin A, Alekseev A, Rodriguez-Valera F, Borshchevskiy V, Cherezov V, Leonard GA, Gordeliy V, Popov A Sci Adv. 2017 May 12;3(5):e1602952. doi: 10.1126/sciadv.1602952. eCollection 2017, May. PMID:28508075[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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