5jm0: Difference between revisions

Latest revision as of 21:58, 20 September 2023

Structure of the S. cerevisiae alpha-mannosidase 1Structure of the S. cerevisiae alpha-mannosidase 1

5jm0, resolution 6.30Å

Structural highlights

5jm0 is a 1 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 6.3Å
Experimental data:	Check
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MAN1_YEAST Degrades free oligosaccharides in the vacuole.^[1]

Publication Abstract from PubMed

Selective autophagy is the mechanism by which large cargos are specifically sequestered for degradation. The structural details of cargo and receptor assembly giving rise to autophagic vesicles remain to be elucidated. We utilize the yeast cytoplasm-to-vacuole targeting (Cvt) pathway, a prototype of selective autophagy, together with a multi-scale analysis approach to study the molecular structure of Cvt vesicles. We report the oligomeric nature of the major Cvt cargo Ape1 with a combined 2.8 A X-ray and negative stain EM structure, as well as the secondary cargo Ams1 with a 6.3 A cryo-EM structure. We show that the major dodecameric cargo prApe1 exhibits a tendency to form higher-order chain structures that are broken upon interaction with the receptor Atg19 in vitro The stoichiometry of these cargo-receptor complexes is key to maintaining the size of the Cvt aggregate in vivo Using correlative light and electron microscopy, we further visualize key stages of Cvt vesicle biogenesis. Our findings suggest that Atg19 interaction limits Ape1 aggregate size while serving as a vehicle for vacuolar delivery of tetrameric Ams1.

Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle.,Bertipaglia C, Schneider S, Jakobi AJ, Tarafder AK, Bykov YS, Picco A, Kukulski W, Kosinski J, Hagen WJ, Ravichandran AC, Wilmanns M, Kaksonen M, Briggs JA, Sachse C EMBO Rep. 2016 Jun 6. pii: e201541960. PMID:27266708^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chantret I, Frenoy JP, Moore SE. Free-oligosaccharide control in the yeast Saccharomyces cerevisiae: roles for peptide:N-glycanase (Png1p) and vacuolar mannosidase (Ams1p). Biochem J. 2003 Aug 1;373(Pt 3):901-8. PMID:12723970 doi:http://dx.doi.org/10.1042/BJ20030384
↑ Bertipaglia C, Schneider S, Jakobi AJ, Tarafder AK, Bykov YS, Picco A, Kukulski W, Kosinski J, Hagen WJ, Ravichandran AC, Wilmanns M, Kaksonen M, Briggs JA, Sachse C. Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle. EMBO Rep. 2016 Jun 6. pii: e201541960. PMID:27266708 doi:http://dx.doi.org/10.15252/embr.201541960

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OCA

[1] Chantret I, Frenoy JP, Moore SE. Free-oligosaccharide control in the yeast Saccharomyces cerevisiae: roles for peptide:N-glycanase (Png1p) and vacuolar mannosidase (Ams1p). Biochem J. 2003 Aug 1;373(Pt 3):901-8. PMID:12723970 doi:http://dx.doi.org/10.1042/BJ20030384

[2] Bertipaglia C, Schneider S, Jakobi AJ, Tarafder AK, Bykov YS, Picco A, Kukulski W, Kosinski J, Hagen WJ, Ravichandran AC, Wilmanns M, Kaksonen M, Briggs JA, Sachse C. Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle. EMBO Rep. 2016 Jun 6. pii: e201541960. PMID:27266708 doi:http://dx.doi.org/10.15252/embr.201541960

[1]

[2]

@@ Line 3: / Line 3: @@
 <SX load='5jm0' size='340' side='right' viewer='molstar' caption='[[5jm0]], [[Resolution|resolution]] 6.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5jm0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JM0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5JM0 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5jm0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JM0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JM0 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 6.3&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AMS1, YGL156W, G1861 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jm0 OCA], [https://pdbe.org/5jm0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jm0 RCSB], [https://www.ebi.ac.uk/pdbsum/5jm0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jm0 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5jm0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jm0 OCA], [http://pdbe.org/5jm0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jm0 RCSB], [http://www.ebi.ac.uk/pdbsum/5jm0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jm0 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/MAN1_YEAST MAN1_YEAST] Degrades free oligosaccharides in the vacuole.<ref>PMID:12723970</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 25: @@
 __TOC__
 </SX>
-[[Category: Baker's yeast]]
 [[Category: Large Structures]]
-[[Category: Hagen, W J.H]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Jakobi, A J]]
+[[Category: Hagen WJH]]
-[[Category: Kosinski, J]]
+[[Category: Jakobi AJ]]
-[[Category: Sachse, C]]
+[[Category: Kosinski J]]
-[[Category: Schneider, S]]
+[[Category: Sachse C]]
-[[Category: Cvt cargo]]
+[[Category: Schneider S]]
-[[Category: Hydrolase]]
-[[Category: Mannosidase]]
-[[Category: Selective autophagy]]
-[[Category: Tetramer]]

5jm0: Difference between revisions

Latest revision as of 21:58, 20 September 2023

Structure of the S. cerevisiae alpha-mannosidase 1Structure of the S. cerevisiae alpha-mannosidase 1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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5jm0: Difference between revisions

Latest revision as of 21:58, 20 September 2023

Structure of the S. cerevisiae alpha-mannosidase 1Structure of the S. cerevisiae alpha-mannosidase 1

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Search