8h2f: Difference between revisions
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==Crystal structure of DnaQ domain in complex witn TMP of Streptococcus thermophilus strain DGCC 7710== | |||
<StructureSection load='8h2f' size='340' side='right'caption='[[8h2f]], [[Resolution|resolution]] 1.45Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8h2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_DGCC_7710 Streptococcus thermophilus DGCC 7710]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8H2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8H2F FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.448Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8h2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8h2f OCA], [https://pdbe.org/8h2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8h2f RCSB], [https://www.ebi.ac.uk/pdbsum/8h2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8h2f ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In the spacer acquisition stage of CRISPR-Cas immunity, spacer orientation and protospacer adjacent motif (PAM) removal are two prerequisites for functional spacer integration. Cas4 has been implicated in both processing the prespacer and determining the spacer orientation. In Cas4-lacking systems, host 3'-5' DnaQ family exonucleases were recently reported to play a Cas4-like role. However, the molecular details of DnaQ functions remain elusive. Here, we characterized the spacer acquisition of the adaptation module of the Streptococcus thermophilus type I-E system, in which a DnaQ domain naturally fuses with Cas2. We presented X-ray crystal structures and cryo-electron microscopy structures of this adaptation module. Our biochemical data showed that DnaQ trimmed PAM-containing and PAM-deficient overhangs with different efficiencies. Based on these results, we proposed a time-dependent model for DnaQ-mediated spacer acquisition to elucidate PAM removal and spacer orientation determination in Cas4-lacking CRISPR-Cas systems. | |||
DnaQ mediates directional spacer acquisition in the CRISPR-Cas system by a time-dependent mechanism.,Tang D, Jia T, Luo Y, Mou B, Cheng J, Qi S, Yao S, Su Z, Yu Y, Chen Q Innovation (Camb). 2023 Aug 9;4(5):100495. doi: 10.1016/j.xinn.2023.100495. , eCollection 2023 Sep 11. PMID:37663930<ref>PMID:37663930</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 8h2f" style="background-color:#fffaf0;"></div> | ||
[[Category: Chen | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptococcus thermophilus DGCC 7710]] | |||
[[Category: Chen Q]] | |||
[[Category: Yu Y]] | |||
Revision as of 21:15, 20 September 2023
Crystal structure of DnaQ domain in complex witn TMP of Streptococcus thermophilus strain DGCC 7710Crystal structure of DnaQ domain in complex witn TMP of Streptococcus thermophilus strain DGCC 7710
Structural highlights
Publication Abstract from PubMedIn the spacer acquisition stage of CRISPR-Cas immunity, spacer orientation and protospacer adjacent motif (PAM) removal are two prerequisites for functional spacer integration. Cas4 has been implicated in both processing the prespacer and determining the spacer orientation. In Cas4-lacking systems, host 3'-5' DnaQ family exonucleases were recently reported to play a Cas4-like role. However, the molecular details of DnaQ functions remain elusive. Here, we characterized the spacer acquisition of the adaptation module of the Streptococcus thermophilus type I-E system, in which a DnaQ domain naturally fuses with Cas2. We presented X-ray crystal structures and cryo-electron microscopy structures of this adaptation module. Our biochemical data showed that DnaQ trimmed PAM-containing and PAM-deficient overhangs with different efficiencies. Based on these results, we proposed a time-dependent model for DnaQ-mediated spacer acquisition to elucidate PAM removal and spacer orientation determination in Cas4-lacking CRISPR-Cas systems. DnaQ mediates directional spacer acquisition in the CRISPR-Cas system by a time-dependent mechanism.,Tang D, Jia T, Luo Y, Mou B, Cheng J, Qi S, Yao S, Su Z, Yu Y, Chen Q Innovation (Camb). 2023 Aug 9;4(5):100495. doi: 10.1016/j.xinn.2023.100495. , eCollection 2023 Sep 11. PMID:37663930[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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