4n7c: Difference between revisions
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<StructureSection load='4n7c' size='340' side='right'caption='[[4n7c]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='4n7c' size='340' side='right'caption='[[4n7c]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4n7c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4n7c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Blattella_germanica Blattella germanica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4N7C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4N7C FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AEF:4-(2-AMINOETHYL)PHENOL'>AEF</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AEF:4-(2-AMINOETHYL)PHENOL'>AEF</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7c OCA], [https://pdbe.org/4n7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n7c RCSB], [https://www.ebi.ac.uk/pdbsum/4n7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n7c ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4n7c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4n7c OCA], [https://pdbe.org/4n7c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4n7c RCSB], [https://www.ebi.ac.uk/pdbsum/4n7c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4n7c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/B7TYB2_BLAGE B7TYB2_BLAGE] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Blattella germanica]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Chan | [[Category: Chan SL]] | ||
[[Category: Chew | [[Category: Chew FT]] | ||
[[Category: Chruszcz | [[Category: Chruszcz M]] | ||
[[Category: Minor | [[Category: Minor W]] | ||
[[Category: Mok | [[Category: Mok YK]] | ||
[[Category: Offermann | [[Category: Offermann LR]] | ||
[[Category: Osinski | [[Category: Osinski T]] | ||
[[Category: Sivaraman | [[Category: Sivaraman J]] | ||
[[Category: Tan | [[Category: Tan YW]] | ||
Latest revision as of 19:48, 20 September 2023
Structural re-examination of native Bla g 4Structural re-examination of native Bla g 4
Structural highlights
FunctionPublication Abstract from PubMedBla g 4 is a male cockroach specific protein and is one of the major allergens produced by Blattella germanica (German cockroach). This protein belongs to the lipocalin family that comprises a set of proteins that characteristically bind small hydrophobic molecules and play a role in a number of processes such as: retinoid and pheromone transport, prostaglandin synthesis and mammalian immune response. Using NMR and isothermal titration calorimetry we demonstrated that Bla g 4 binds tyramine and octopamine in solution. In addition, crystal structure analysis of the complex revealed details of tyramine binding. As tyramine and octopamine play important roles in invertebrates, and are counterparts to vertebrate adrenergic transmitters, we speculate that these molecules are physiological ligands for Bla g 4. The nature of binding these ligands to Bla g 4 sheds light on the possible biological function of the protein. In addition, we performed a large-scale analysis of Bla g 4 and Per a 4 (an allergen from American cockroach) homologs to get insights into the function of these proteins. This analysis together with a structural comparison of Blag 4 and Per a 4 suggests that these proteins may play different roles and most likely bind different ligands. Accession numbers: The atomic coordinates and the structure factors have been deposited to the Protein Data Band under accession codes: 4N7C for native Bla g 4 and 4N7D for the Se-Met Bla g 4 structure. The major cockroach allergen Bla g 4 binds tyramine and octopamine.,Offermann LR, Chan SL, Osinski T, Tan YW, Chew FT, Sivaraman J, Mok YK, Minor W, Chruszcz M Mol Immunol. 2014 Jul;60(1):86-94. doi: 10.1016/j.molimm.2014.03.016. Epub 2014, Apr 24. PMID:24769496[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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