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==Refinement of RNase P P4 stemloop structure using residual dipolar coupling data - C70U mutant==
==Refinement of RNase P P4 stemloop structure using residual dipolar coupling data - C70U mutant==
<StructureSection load='2cd3' size='340' side='right'caption='[[2cd3]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
<StructureSection load='2cd3' size='340' side='right'caption='[[2cd3]]' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2cd3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CD3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2cd3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CD3 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2cd1|2cd1]], [[2cd5|2cd5]], [[2cd6|2cd6]]</div></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd3 OCA], [https://pdbe.org/2cd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cd3 RCSB], [https://www.ebi.ac.uk/pdbsum/2cd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cd3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cd3 OCA], [https://pdbe.org/2cd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cd3 RCSB], [https://www.ebi.ac.uk/pdbsum/2cd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cd3 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We determined the solution structure of two 27-nt RNA hairpins and their complexes with cobalt(III)-hexammine (Co(NH3)3+(6)) by NMR spectroscopy. The RNA hairpins used in this study are the P4 region from Escherichia coli RNase P RNA and a C-to-U mutant that confers altered divalent metal-ion specificity (Ca2+ replaces Mg2+) for catalytic activity of this ribozyme. Co(NH3)3+(6) is a useful spectroscopic probe for Mg(H2O)2+(6)-binding sites because both complexes have octahedral symmetry and have similar radii. The thermodynamics of binding to both RNA hairpins was studied using chemical shift changes upon titration with Mg2+, Ca2+, and Co(NH3)3+(6). We found that the equilibrium binding constants for each of the metal ions was essentially unchanged when the P4 model RNA hairpin was mutated, although the NMR structures show that the RNA hairpins adopt different conformations. In the C-to-U mutant a C.G base pair is replaced by U.G, and the conserved bulged uridine in the P4 wild-type stem shifts in the 3' direction by 1 nt. Intermolecular NOE cross-peaks between Co(NH3)3+(6) and RNA protons were used to locate the site of Co(NH3)3+(6) binding to both RNA hairpins. The metal ion binds in the major groove near a bulge loop, but is shifted 5' by more than 1 bp in the mutant. The change of the metal-ion binding site provides a possible explanation for changes in catalytic activity of the mutant RNase P in the presence of Ca2+.
Solution structure and metal-ion binding of the P4 element from bacterial RNase P RNA.,Schmitz M, Tinoco I Jr RNA. 2000 Sep;6(9):1212-25. PMID:10999599<ref>PMID:10999599</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2cd3" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Ribozyme|Ribozyme]]
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Schmitz, M]]
[[Category: Schmitz M]]
[[Category: C70u mutant]]
[[Category: Metal binding site]]
[[Category: Nucleic acid]]
[[Category: P4 stem]]
[[Category: Ribonuclease p]]
[[Category: Ribonucleic acid]]
[[Category: Ribozyme]]
[[Category: Transfer rna processing]]

Latest revision as of 13:25, 6 September 2023

Refinement of RNase P P4 stemloop structure using residual dipolar coupling data - C70U mutantRefinement of RNase P P4 stemloop structure using residual dipolar coupling data - C70U mutant

Structural highlights

2cd3 is a 1 chain structure with sequence from Escherichia coli. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

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