3h0v: Difference between revisions
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<StructureSection load='3h0v' size='340' side='right'caption='[[3h0v]], [[Resolution|resolution]] 2.24Å' scene=''> | <StructureSection load='3h0v' size='340' side='right'caption='[[3h0v]], [[Resolution|resolution]] 2.24Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3h0v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3h0v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3H0V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3H0V FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M2T:5-DEOXY-5-(DIMETHYL-LAMBDA~4~-SULFANYL)ADENOSINE'>M2T</scene>, <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene>, <scene name='pdbligand=PYR:PYRUVIC+ACID'>PYR</scene></td></tr> | |||
<tr id=' | |||
< | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h0v OCA], [https://pdbe.org/3h0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h0v RCSB], [https://www.ebi.ac.uk/pdbsum/3h0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h0v ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3h0v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3h0v OCA], [https://pdbe.org/3h0v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3h0v RCSB], [https://www.ebi.ac.uk/pdbsum/3h0v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3h0v ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/DCAM_HUMAN DCAM_HUMAN] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bale | [[Category: Bale S]] | ||
[[Category: Brooks | [[Category: Brooks WH]] | ||
[[Category: Ealick | [[Category: Ealick SE]] | ||
[[Category: Guida | [[Category: Guida WC]] | ||
[[Category: Hanes | [[Category: Hanes JW]] | ||
[[Category: Mahesan | [[Category: Mahesan AM]] | ||
Latest revision as of 10:14, 6 September 2023
Human AdoMetDC with 5'-Deoxy-5'-(dimethylsulfonio) adenosineHuman AdoMetDC with 5'-Deoxy-5'-(dimethylsulfonio) adenosine
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedS-Adenosylmethionine decarboxylase (AdoMetDC) is a key enzyme in the polyamine biosynthetic pathway. Inhibition of this pathway and subsequent depletion of polyamine levels is a viable strategy for cancer chemotherapy and for the treatment of parasitic diseases. Substrate analogue inhibitors display an absolute requirement for a positive charge at the position equivalent to the sulfonium of S-adenosylmethionine. We investigated the ligand specificity of AdoMetDC through crystallography, quantum chemical calculations, and stopped-flow experiments. We determined crystal structures of the enzyme cocrystallized with 5'-deoxy-5'-dimethylthioadenosine and 5'-deoxy-5'-(N-dimethyl)amino-8-methyladenosine. The crystal structures revealed a favorable cation-pi interaction between the ligand and the aromatic side chains of Phe7 and Phe223. The estimated stabilization from this interaction is 4.5 kcal/mol as determined by quantum chemical calculations. Stopped-flow kinetic experiments showed that the rate of the substrate binding to the enzyme greatly depends on Phe7 and Phe223, thus supporting the importance of the cation-pi interaction. Role of the Sulfonium Center in Determining the Ligand Specificity of Human S-Adenosylmethionine Decarboxylase.,Bale S, Brooks W, Hanes JW, Mahesan AM, Guida WC, Ealick SE Biochemistry. 2009 Jun 15. PMID:19527050[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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