1mme: Difference between revisions
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'''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE''' | '''THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE''' | ||
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==About this Structure== | ==About this Structure== | ||
The following page contains interesting information on the relation of 1MME with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb65_1.html Self-splicing RNA]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MME OCA]. | |||
==Reference== | ==Reference== | ||
The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7541315 7541315] | The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7541315 7541315] | ||
[[Category: Self-splicing RNA]] | [[Category: Self-splicing RNA]] | ||
[[Category: Finch, J T.]] | [[Category: Finch, J T.]] | ||
[[Category: Klug, A.]] | [[Category: Klug, A.]] | ||
[[Category: Scott, W G.]] | [[Category: Scott, W G.]] | ||
[[Category: | [[Category: Catalytic rna]] | ||
[[Category: | [[Category: Loop]] | ||
[[Category: | [[Category: Rna hammerhead ribozyme]] | ||
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Revision as of 01:25, 3 May 2008
THE CRYSTAL STRUCTURE OF AN ALL-RNA HAMMERHEAD RIBOZYME: A PROPOSED MECHANISM FOR RNA CATALYTIC CLEAVAGE
OverviewOverview
We have solved the crystal structure of an all-RNA hammerhead ribozyme having a single 2'-O-methyl cytosine incorporated at the active site to prevent cleavage. The conditions used differ from those in another recent solution in four significant ways: first, it is an all-RNA ribozyme rather than a DNA-RNA hybrid; second, the connectivity of the ribozyme backbone strands is different; third, the crystals were grown in the presence of a much lower concentration of salt; and fourth, the crystal packing scheme is very different. Nevertheless, the three-dimensional structure of the all-RNA hammerhead ribozyme is similar to the previous structure. Five potential Mg(II)-binding sites are identified, including one positioned near the ribozyme catalytic pocket. Upon this basis, as well as upon comparisons with the metal-binding sites in the structurally homologous uridine turn of tRNAPhe, we propose a mechanism for RNA catalytic cleavage.
About this StructureAbout this Structure
The following page contains interesting information on the relation of 1MME with [Self-splicing RNA]. Full crystallographic information is available from OCA.
ReferenceReference
The crystal structure of an all-RNA hammerhead ribozyme: a proposed mechanism for RNA catalytic cleavage., Scott WG, Finch JT, Klug A, Cell. 1995 Jun 30;81(7):991-1002. PMID:7541315 Page seeded by OCA on Sat May 3 01:25:02 2008