1ml9: Difference between revisions

Revision as of 01:22, 3 May 2008

Structure of the Neurospora SET domain protein DIM-5, a histone lysine methyltransferase

OverviewOverview

AdoMet-dependent methylation of histones is part of the "histone code" that can profoundly influence gene expression. We describe the crystal structure of Neurospora DIM-5, a histone H3 lysine 9 methyltranferase (HKMT), determined at 1.98 A resolution, as well as results of biochemical characterization and site-directed mutagenesis of key residues. This SET domain protein bears no structural similarity to previously characterized AdoMet-dependent methyltransferases but includes notable features such as a triangular Zn3Cys9 zinc cluster in the pre-SET domain and a AdoMet binding site in the SET domain essential for methyl transfer. The structure suggests a mechanism for the methylation reaction and provides the structural basis for functional characterization of the HKMT family and the SET domain.

About this StructureAbout this Structure

1ML9 is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the Neurospora SET domain protein DIM-5, a histone H3 lysine methyltransferase., Zhang X, Tamaru H, Khan SI, Horton JR, Keefe LJ, Selker EU, Cheng X, Cell. 2002 Oct 4;111(1):117-27. PMID:12372305 Page seeded by OCA on Sat May 3 01:22:26 2008

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OCA

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 [[Image:1ml9.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ml9 |SIZE=350|CAPTION= <scene name='initialview01'>1ml9</scene>, resolution 1.98&Aring;
+The line below this paragraph, containing "STRUCTURE_1ml9", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ml9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ml9 OCA], [http://www.ebi.ac.uk/pdbsum/1ml9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ml9 RCSB]</span>
-}}
 '''Structure of the Neurospora SET domain protein DIM-5, a histone lysine methyltransferase'''
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 [[Category: Tamaru, H.]]
 [[Category: Zhang, X.]]
-[[Category: adomet-dependent methyltransferase histone h3 lysine-9 methylation]]
+[[Category: Adomet-dependent methyltransferase histone h3 lysine-9 methylation]]
-[[Category: dim-5]]
+[[Category: Dim-5]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:22:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:17:34 2008''