3dw4: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3dw4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DW4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[3dw4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DW4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DW4 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.97Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw4 OCA], [https://pdbe.org/3dw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dw4 RCSB], [https://www.ebi.ac.uk/pdbsum/3dw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw4 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dw4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw4 OCA], [https://pdbe.org/3dw4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dw4 RCSB], [https://www.ebi.ac.uk/pdbsum/3dw4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Dumas | [[Category: Dumas P]] | ||
[[Category: Ennifar | [[Category: Ennifar E]] | ||
[[Category: Lang | [[Category: Lang K]] | ||
[[Category: Micura | [[Category: Micura R]] | ||
[[Category: Olieric | [[Category: Olieric V]] | ||
[[Category: Rieder | [[Category: Rieder U]] | ||
[[Category: Schulze-Briese | [[Category: Schulze-Briese C]] | ||
[[Category: Serganov | [[Category: Serganov A]] | ||
Latest revision as of 15:53, 30 August 2023
Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modifiedCrystal Structure of the Sarcin/Ricin Domain from E. COLI 23 S rRNA, U2650-OCH3 modified
Structural highlights
Publication Abstract from PubMedSite-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality. A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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