1miu: Difference between revisions

Revision as of 01:07, 3 May 2008

Structure of a BRCA2-DSS1 complex

OverviewOverview

Mutations in the BRCA2 (breast cancer susceptibility gene 2) tumor suppressor lead to chromosomal instability due to defects in the repair of double-strand DNA breaks (DSBs) by homologous recombination, but BRCA2's role in this process has been unclear. Here, we present the 3.1 angstrom crystal structure of a approximately 90-kilodalton BRCA2 domain bound to DSS1, which reveals three oligonucleotide-binding (OB) folds and a helix-turn-helix (HTH) motif. We also (i) demonstrate that this BRCA2 domain binds single-stranded DNA, (ii) present its 3.5 angstrom structure bound to oligo(dT)9, (iii) provide data that implicate the HTH motif in dsDNA binding, and (iv) show that BRCA2 stimulates RAD51-mediated recombination in vitro. These findings establish that BRCA2 functions directly in homologous recombination and provide a structural and biochemical basis for understanding the loss of recombination-mediated DSB repair in BRCA2-associated cancers.

About this StructureAbout this Structure

1MIU is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure., Yang H, Jeffrey PD, Miller J, Kinnucan E, Sun Y, Thoma NH, Zheng N, Chen PL, Lee WH, Pavletich NP, Science. 2002 Sep 13;297(5588):1837-48. PMID:12228710 Page seeded by OCA on Sat May 3 01:07:51 2008

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OCA

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 [[Image:1miu.gif|left|200px]]
- {{Structure
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 '''Structure of a BRCA2-DSS1 complex'''
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 [[Category: Yang, H.]]
 [[Category: Zheng, N.]]
-[[Category: breast cancer susceptibility]]
+[[Category: Breast cancer susceptibility]]
-[[Category: dna-binding]]
+[[Category: Dna-binding]]
-[[Category: tumor suppressor]]
+[[Category: Tumor suppressor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:07:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:39 2008''