1meg: Difference between revisions

Revision as of 00:56, 3 May 2008

CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64

OverviewOverview

The structure of the D158E mutant of caricain (previously known as papaya protease omega) in complex with E-64 has been determined at 2.0 A resolution (overall R factor 19.3%). The structure reveals that the substituted glutamate makes the same pattern of hydrogen bonds as the aspartate in native caricain. This was not anticipated since in the native structure there is insufficient room to accommodate the glutamate side chain. The glutamate is accommodated in the mutant by a local expansion of the structure demonstrating that small structural changes are responsible for the change in activity.

About this StructureAbout this Structure

1MEG is a Single protein structure of sequence from Carica papaya. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a caricain D158E mutant in complex with E-64., Katerelos NA, Taylor MA, Scott M, Goodenough PW, Pickersgill RW, FEBS Lett. 1996 Aug 19;392(1):35-9. PMID:8769310 Page seeded by OCA on Sat May 3 00:56:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1meg.jpg|left|200px]]
- {{Structure
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Caricain Caricain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.30 3.4.22.30] </span>
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-}}
 '''CRYSTAL STRUCTURE OF A CARICAIN D158E MUTANT IN COMPLEX WITH E-64'''
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 [[Category: Single protein]]
 [[Category: Katerelos, N A.]]
-[[Category: cysteine proteinase]]
+[[Category: Cysteine proteinase]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: thiol protease]]
+[[Category: Thiol protease]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:56:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:15:06 2008''