1md0: Difference between revisions
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'''CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1''' | '''CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1''' | ||
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[[Category: Pufall, M A.]] | [[Category: Pufall, M A.]] | ||
[[Category: Wolberger, C.]] | [[Category: Wolberger, C.]] | ||
[[Category: | [[Category: Autoinhibition]] | ||
[[Category: | [[Category: Transcription factor]] | ||
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Revision as of 00:53, 3 May 2008
CRYSTAL STRUCTURE OF AN INHIBITED FRAGMENT OF Ets-1
OverviewOverview
The DNA-binding activity of the eukaryotic transcription factor Ets-1 (E26 avian erythroblastosis virus oncogene-E twenty-six) is negatively regulated by inhibitory regions that flank the ETS domain. Based on the results of solution studies, these N- and C-terminal inhibitory regions have been proposed to pack against the ETS domain and form an autoinhibitory module whose N terminus partially unfolds upon binding of Ets-1 to DNA. Mutations that disrupt autoinhibition of DNA binding also cause a structural change in the inhibitory region. We report here a crystallographic study of fragments of Ets-1 that provide structural details of the inhibitory module and the structural transition that accompanies DNA binding. The structures of free and DNA-bound Ets-1 fragments containing the ETS domain and the inhibitory regions confirm that the N-terminal inhibitory region contains two alpha-helices one of which unfolds upon Ets-1 binding to DNA. The observations from the crystal structure, coupled with mutagenesis experiments, allow us to propose a model for the inhibited form of Ets-1 and lend insight into the flexible interaction between Ets-1 and the acute myeloid leukemia 1 protein, AML1 (RUNX1).
About this StructureAbout this Structure
1MD0 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structural analysis of the autoinhibition of Ets-1 and its role in protein partnerships., Garvie CW, Pufall MA, Graves BJ, Wolberger C, J Biol Chem. 2002 Nov 22;277(47):45529-36. Epub 2002 Sep 6. PMID:12221090 Page seeded by OCA on Sat May 3 00:53:58 2008