2y8s: Difference between revisions
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<StructureSection load='2y8s' size='340' side='right'caption='[[2y8s]], [[Resolution|resolution]] 2.55Å' scene=''> | <StructureSection load='2y8s' size='340' side='right'caption='[[2y8s]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2y8s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2y8s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Y8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Y8S FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BO3:BORIC+ACID'>BO3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BO3:BORIC+ACID'>BO3</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8s OCA], [https://pdbe.org/2y8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8s RCSB], [https://www.ebi.ac.uk/pdbsum/2y8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8s ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2y8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2y8s OCA], [https://pdbe.org/2y8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2y8s RCSB], [https://www.ebi.ac.uk/pdbsum/2y8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2y8s ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/AMA1_TOXGV AMA1_TOXGV] Essential microneme protein that plays an important role in host cell invasion. Part of the moving junction (MJ) complex, a ringlike structure formed between the plasma membranes of the apical tip of the parasite and the target host cell. During invasion, the MJ migrates from the anterior to the posterior of the parasite, leading to internalization of the parasite into a parasitophorous vacuole (PV).<ref>PMID:11083833</ref> <ref>PMID:11087913</ref> <ref>PMID:16000372</ref> <ref>PMID:16102004</ref> <ref>PMID:16244709</ref> <ref>PMID:19247437</ref> <ref>PMID:20421941</ref> <ref>PMID:21205639</ref> <ref>PMID:22523242</ref> | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Toxoplasma gondii]] | ||
[[Category: Boulanger | [[Category: Boulanger MJ]] | ||
[[Category: Crawford | [[Category: Crawford J]] | ||
[[Category: Douguet | [[Category: Douguet D]] | ||
[[Category: Lamarque | [[Category: Lamarque MH]] | ||
[[Category: Lebrun | [[Category: Lebrun M]] | ||
[[Category: Pugniere | [[Category: Pugniere M]] | ||
[[Category: Roques | [[Category: Roques M]] | ||
[[Category: Tonkin | [[Category: Tonkin ML]] | ||
Latest revision as of 11:10, 23 August 2023
Co-structure of an AMA1 mutant (Y230A) with a surface exposed region of RON2 from Toxoplasma gondiiCo-structure of an AMA1 mutant (Y230A) with a surface exposed region of RON2 from Toxoplasma gondii
Structural highlights
FunctionAMA1_TOXGV Essential microneme protein that plays an important role in host cell invasion. Part of the moving junction (MJ) complex, a ringlike structure formed between the plasma membranes of the apical tip of the parasite and the target host cell. During invasion, the MJ migrates from the anterior to the posterior of the parasite, leading to internalization of the parasite into a parasitophorous vacuole (PV).[1] [2] [3] [4] [5] [6] [7] [8] [9] Publication Abstract from PubMedApicomplexan parasites such as Toxoplasma gondii and Plasmodium species actively invade host cells through a moving junction (MJ) complex assembled at the parasite-host cell interface. MJ assembly is initiated by injection of parasite rhoptry neck proteins (RONs) into the host cell, where RON2 spans the membrane and functions as a receptor for apical membrane antigen 1 (AMA1) on the parasite. We have determined the structure of TgAMA1 complexed with a RON2 peptide at 1.95 angstrom resolution. A stepwise assembly mechanism results in an extensive buried surface area, enabling the MJ complex to resist the mechanical forces encountered during host cell invasion. Besides providing insights into host cell invasion by apicomplexan parasites, the structure offers a basis for designing therapeutics targeting these global pathogens. Host cell invasion by apicomplexan parasites: insights from the co-structure of AMA1 with a RON2 peptide.,Tonkin ML, Roques M, Lamarque MH, Pugniere M, Douguet D, Crawford J, Lebrun M, Boulanger MJ Science. 2011 Jul 22;333(6041):463-7. PMID:21778402[10] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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