2xpi: Difference between revisions
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<StructureSection load='2xpi' size='340' side='right'caption='[[2xpi]], [[Resolution|resolution]] 2.60Å' scene=''> | <StructureSection load='2xpi' size='340' side='right'caption='[[2xpi]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2xpi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2xpi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XPI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XPI FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AUC:GOLD+(I)+CYANIDE+ION'>AUC</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xpi OCA], [https://pdbe.org/2xpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xpi RCSB], [https://www.ebi.ac.uk/pdbsum/2xpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xpi ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xpi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xpi OCA], [https://pdbe.org/2xpi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xpi RCSB], [https://www.ebi.ac.uk/pdbsum/2xpi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xpi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/CUT9_SCHPO CUT9_SCHPO] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: | [[Category: Schizosaccharomyces pombe]] | ||
[[Category: | [[Category: Barford D]] | ||
[[Category: | [[Category: Kulkarni KA]] | ||
[[Category: | [[Category: Zhang Z]] | ||
Latest revision as of 11:02, 23 August 2023
Crystal structure of APC/C hetero-tetramer Cut9-Hcn1Crystal structure of APC/C hetero-tetramer Cut9-Hcn1
Structural highlights
FunctionPublication Abstract from PubMedThe anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase responsible for controlling cell cycle transitions, is a multisubunit complex assembled from 13 different proteins. Numerous APC/C subunits incorporate multiple copies of the tetratricopeptide repeat (TPR). Here, we report the crystal structure of Schizosaccharomyces pombe Cut9 (Cdc16/Apc6) in complex with Hcn1 (Cdc26), showing that Cdc16/Cut9 is a contiguous TPR superhelix of 14 TPR units. A C-terminal block of TPR motifs interacts with Hcn1, whereas an N-terminal TPR block mediates Cdc16/Cut9 self-association through a homotypic interface. This dimer interface is structurally related to the N-terminal dimerization domain of Cdc27, demonstrating that both Cdc16/Cut9 and Cdc27 form homo-dimers through a conserved mechanism. The acetylated N-terminal Met residue of Hcn1 is enclosed within a chamber created from the Cut9 TPR superhelix. Thus, in complex with Cdc16/Cut9, the N-acetyl-Met residue of Hcn1, a putative degron for the Doa10 E3 ubiquitin ligase, is inaccessible for Doa10 recognition, protecting Hcn1/Cdc26 from ubiquitin-dependent degradation. This finding may provide a structural explanation for a mechanism to control the stoichiometry of proteins participating in multisubunit complexes. The APC/C subunit Cdc16/Cut9 is a contiguous tetratricopeptide repeat superhelix with a homo-dimer interface similar to Cdc27.,Zhang Z, Kulkarni K, Hanrahan SJ, Thompson AJ, Barford D EMBO J. 2010 Nov 3;29(21):3733-44. Epub 2010 Oct 5. PMID:20924356[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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