1m90: Difference between revisions

Revision as of 00:46, 3 May 2008

Co-crystal structure of CCA-Phe-caproic acid-biotin and sparsomycin bound to the 50S ribosomal subunit

OverviewOverview

The large ribosomal subunit catalyzes peptide bond formation and will do so by using small aminoacyl- and peptidyl-RNA fragments of tRNA. We have refined at 3-A resolution the structures of both A and P site substrate and product analogues, as well as an intermediate analogue, bound to the Haloarcula marismortui 50S ribosomal subunit. A P site substrate, CCA-Phe-caproic acid-biotin, binds equally to both sites, but in the presence of sparsomycin binds only to the P site. The CCA portions of these analogues are bound identically by either the A or P loop of the 23S rRNA. Combining the separate P and A site substrate complexes into one model reveals interactions that may occur when both are present simultaneously. The alpha-NH(2) group of an aminoacylated fragment in the A site forms one hydrogen bond with the N3 of A2486 (2451) and may form a second hydrogen bond either with the 2' OH of the A-76 ribose in the P site or with the 2' OH of A2486 (2451). These interactions position the alpha amino group adjacent to the carbonyl carbon of esterified P site substrate in an orientation suitable for a nucleophilic attack.

About this StructureAbout this Structure

1M90 is a Protein complex structure of sequences from Haloarcula marismortui. Full crystallographic information is available from OCA.

ReferenceReference

Structural insights into peptide bond formation., Hansen JL, Schmeing TM, Moore PB, Steitz TA, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11670-5. Epub 2002 Aug 16. PMID:12185246 Page seeded by OCA on Sat May 3 00:46:51 2008

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OCA

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 [[Image:1m90.gif|left|200px]]
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m90 OCA], [http://www.ebi.ac.uk/pdbsum/1m90 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m90 RCSB]</span>
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 '''Co-crystal structure of CCA-Phe-caproic acid-biotin and sparsomycin bound to the 50S ribosomal subunit'''
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 [[Category: Schmeing, T M.]]
 [[Category: Steitz, T A.]]
-[[Category: p-site]]
+[[Category: P-site]]
-[[Category: ribosome]]
+[[Category: Ribosome]]
-[[Category: sparsomycin]]
+[[Category: Sparsomycin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:46:51 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:13:02 2008''