1s8g: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1s8g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agkistrodon_contortrix_laticinctus Agkistrodon contortrix laticinctus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S8G FirstGlance]. <br> | <table><tr><td colspan='2'>[[1s8g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agkistrodon_contortrix_laticinctus Agkistrodon contortrix laticinctus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S8G FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAO:LAURIC+ACID'>DAO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s8g OCA], [https://pdbe.org/1s8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s8g RCSB], [https://www.ebi.ac.uk/pdbsum/1s8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s8g ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s8g OCA], [https://pdbe.org/1s8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s8g RCSB], [https://www.ebi.ac.uk/pdbsum/1s8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s8g ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/PA2H1_AGKCL PA2H1_AGKCL] Snake venom phospholipase A2 homolog that has myotoxic activities. Lacks enzymatic activity. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Agkistrodon contortrix laticinctus]] | [[Category: Agkistrodon contortrix laticinctus]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Ambrosio | [[Category: Ambrosio ALB]] | ||
[[Category: Garratt RC]] | |||
[[Category: Garratt | [[Category: Nonato MC]] | ||
[[Category: Nonato | [[Category: Ownby CL]] | ||
[[Category: Ownby | [[Category: Selistre de Araujo HS]] | ||
[[Category: | [[Category: De Souza DHF]] | ||
[[Category: | |||
Latest revision as of 09:14, 23 August 2023
Crystal structure of Lys49-Phospholipase A2 from Agkistrodon contortrix laticinctus, fatty acid bound formCrystal structure of Lys49-Phospholipase A2 from Agkistrodon contortrix laticinctus, fatty acid bound form
Structural highlights
FunctionPA2H1_AGKCL Snake venom phospholipase A2 homolog that has myotoxic activities. Lacks enzymatic activity. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedAgkistrodon contortrix laticinctus myotoxin is a Lys(49)-phospholipase A(2) (EC 3.1.1.4) isolated from the venom of the serpent A. contortrix laticinctus (broad-banded copperhead). We present here three monomeric crystal structures of the myotoxin, obtained under different crystallization conditions. The three forms present notable structural differences and reveal that the presence of a ligand in the active site (naturally presumed to be a fatty acid) induces the exposure of a hydrophobic surface (the hydrophobic knuckle) toward the C terminus. The knuckle in A. contortrix laticinctus myotoxin involves the side chains of Phe(121) and Phe(124) and is a consequence of the formation of a canonical structure for the main chain within the region of residues 118-125. Comparison with other Lys(49)-phospholipase A(2) myotoxins shows that although the knuckle is a generic structural motif common to all members of the family, it is not readily recognizable by simple sequence analyses. An activation mechanism is proposed that relates fatty acid retention at the active site to conformational changes within the C-terminal region, a part of the molecule that has long been associated with Ca(2+)-independent membrane damaging activity and myotoxicity. This provides, for the first time, a direct structural connection between the phospholipase "active site" and the C-terminal "myotoxic site," justifying the otherwise enigmatic conservation of the residues of the former in supposedly catalytically inactive molecules. A molecular mechanism for Lys49-phospholipase A2 activity based on ligand-induced conformational change.,Ambrosio AL, Nonato MC, de Araujo HS, Arni R, Ward RJ, Ownby CL, de Souza DH, Garratt RC J Biol Chem. 2005 Feb 25;280(8):7326-35. Epub 2004 Dec 13. PMID:15596433[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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