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'''Crystal structure of a Monoclonal Fab Specific for Shigella Flexneri Y lipopolysaccharide''' | '''Crystal structure of a Monoclonal Fab Specific for Shigella Flexneri Y lipopolysaccharide''' | ||
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==About this Structure== | ==About this Structure== | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1M71 OCA]. | |||
==Reference== | ==Reference== | ||
Molecular recognition of oligosaccharide epitopes by a monoclonal Fab specific for Shigella flexneri Y lipopolysaccharide: X-ray structures and thermodynamics., Vyas NK, Vyas MN, Chervenak MC, Johnson MA, Pinto BM, Bundle DR, Quiocho FA, Biochemistry. 2002 Nov 19;41(46):13575-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12427018 12427018] | Molecular recognition of oligosaccharide epitopes by a monoclonal Fab specific for Shigella flexneri Y lipopolysaccharide: X-ray structures and thermodynamics., Vyas NK, Vyas MN, Chervenak MC, Johnson MA, Pinto BM, Bundle DR, Quiocho FA, Biochemistry. 2002 Nov 19;41(46):13575-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12427018 12427018] | ||
[[Category: Bundle, D R.]] | [[Category: Bundle, D R.]] | ||
[[Category: Chervenak, M C.]] | [[Category: Chervenak, M C.]] | ||
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[[Category: Vyas, M N.]] | [[Category: Vyas, M N.]] | ||
[[Category: Vyas, N K.]] | [[Category: Vyas, N K.]] | ||
[[Category: | [[Category: Anti-carbohydrate antibody]] | ||
[[Category: | [[Category: Fab-carbohydrate interaction]] | ||
[[Category: | [[Category: Shigella o-antigen]] | ||
[[Category: | [[Category: X-ray diffracrion]] | ||
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 00:42, 3 May 2008
Crystal structure of a Monoclonal Fab Specific for Shigella Flexneri Y lipopolysaccharide
OverviewOverview
The antigenic recognition of Shigella flexneri O-polysaccharide, which consists of a repeating unit ABCD [-->2)-alpha-L-Rhap-(1-->2)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap-(1-->3)-beta -D-GlcpNAc-(1-->], by the monoclonal antibody SYA/J6 (IgG3, kappa) has been investigated by crystallographic analysis of the Fab domain and its two complexes with two antigen segments (a pentasaccharide Rha A-Rha B-Rha C-GlcNAc D-Rha A' and a modified trisaccharide Rha B-Rha C-GlcNAc D in which Rha C* is missing a C2-OH group). These complex structures, the first for a Fab specific for a periodic linear heteropolysaccharide, reveal a binding site groove (between the V(H) and V(L) domains) that makes polar and nonpolar contacts with all the sugar residues of the pentasaccharide. Both main-chain and side-chain atoms of the Fab are used in ligand binding. The charged side chain of Glu H50 of CDR H2 forms crucial hydrogen bonds to GlcNAc of the oligosaccharides. The modified trisaccharide is more buried and fits more snugly than the pentasaccharide. It also makes as many contacts (approximately 75) with the Fab as the pentasaccharide, including the same number of hydrogen bonds (eight, with four being identical). It is further engaged in more hydrophobic interactions than the pentasaccharide. These three features favorable to trisaccharide binding are consistent with the observation of a tighter complex with the trisaccharide than the pentasaccharide. Thermodynamic data demonstrate that the native tri- to pentasaccharides have free energies of binding in the range of 6.8-7.4 kcal mol(-1), and all but one of the hydrogen bonds to individual hydroxyl groups provide no more than approximately 0.7 kcal mol(-1). They further indicate that hydrophobic interactions make significant contributions to binding and, as the native epitope becomes larger across the tri-, tetra-, pentasaccharide series, entropy contributions to the free energy become dominant.
About this StructureAbout this Structure
Full crystallographic information is available from OCA.
ReferenceReference
Molecular recognition of oligosaccharide epitopes by a monoclonal Fab specific for Shigella flexneri Y lipopolysaccharide: X-ray structures and thermodynamics., Vyas NK, Vyas MN, Chervenak MC, Johnson MA, Pinto BM, Bundle DR, Quiocho FA, Biochemistry. 2002 Nov 19;41(46):13575-86. PMID:12427018 Page seeded by OCA on Sat May 3 00:42:52 2008