8a40: Difference between revisions
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==Structure of mammalian Pol II-TFIIS elongation complex== | |||
<StructureSection load='8a40' size='340' side='right'caption='[[8a40]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8a40]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens], [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8A40 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8A40 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8a40 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8a40 OCA], [https://pdbe.org/8a40 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8a40 RCSB], [https://www.ebi.ac.uk/pdbsum/8a40 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8a40 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A7M4DUC2_PIG A0A7M4DUC2_PIG] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
During gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage when the human elongation factors DSIF, PAF1 complex (PAF), RTF1, SPT6, and TFIIS are present. The cryo-EM structure of an intermediate of the nucleosome passage shows a partially unraveled hexasome that lacks the proximal H2A-H2B dimer and interacts with the RNA Pol II jaw, DSIF, and the CTR9trestle helix. RNA Pol II adopts a backtracked state with the RNA 3' end dislodged from the active site and bound in the RNA Pol II pore. Additional structures and biochemical data show that human TFIIS enters the RNA Pol II pore and stimulates the cleavage of the backtracked RNA and nucleosome passage. | |||
Structure of a backtracked hexasomal intermediate of nucleosome transcription.,Farnung L, Ochmann M, Garg G, Vos SM, Cramer P Mol Cell. 2022 Sep 1;82(17):3126-3134.e7. doi: 10.1016/j.molcel.2022.06.027. Epub , 2022 Jul 19. PMID:35858621<ref>PMID:35858621</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8a40" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Sus scrofa]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Cramer P]] | |||
[[Category: Farnung L]] | |||
[[Category: Garg G]] | |||
[[Category: Ochmann M]] | |||
[[Category: Vos SM]] | |||
Revision as of 08:51, 23 August 2023
Structure of mammalian Pol II-TFIIS elongation complexStructure of mammalian Pol II-TFIIS elongation complex
Structural highlights
FunctionA0A7M4DUC2_PIG DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[RuleBase:RU004279] Publication Abstract from PubMedDuring gene transcription, RNA polymerase II (RNA Pol II) passes nucleosomes with the help of various elongation factors. Here, we show that RNA Pol II achieves efficient nucleosome passage when the human elongation factors DSIF, PAF1 complex (PAF), RTF1, SPT6, and TFIIS are present. The cryo-EM structure of an intermediate of the nucleosome passage shows a partially unraveled hexasome that lacks the proximal H2A-H2B dimer and interacts with the RNA Pol II jaw, DSIF, and the CTR9trestle helix. RNA Pol II adopts a backtracked state with the RNA 3' end dislodged from the active site and bound in the RNA Pol II pore. Additional structures and biochemical data show that human TFIIS enters the RNA Pol II pore and stimulates the cleavage of the backtracked RNA and nucleosome passage. Structure of a backtracked hexasomal intermediate of nucleosome transcription.,Farnung L, Ochmann M, Garg G, Vos SM, Cramer P Mol Cell. 2022 Sep 1;82(17):3126-3134.e7. doi: 10.1016/j.molcel.2022.06.027. Epub , 2022 Jul 19. PMID:35858621[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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