5hrf: Difference between revisions

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<StructureSection load='5hrf' size='340' side='right'caption='[[5hrf]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
<StructureSection load='5hrf' size='340' side='right'caption='[[5hrf]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5hrf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Asf Asf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HRF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5HRF FirstGlance]. <br>
<table><tr><td colspan='2'>[[5hrf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HRF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5hr9|5hr9]], [[5hrb|5hrb]], [[5hrd|5hrd]], [[5hre|5hre]], [[5hrg|5hrg]], [[5hrh|5hrh]], [[5hri|5hri]], [[5hrk|5hrk]], [[5hrl|5hrl]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DGT:2-DEOXYGUANOSINE-5-TRIPHOSPHATE'>DGT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">O174L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10497 ASF])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hrf OCA], [https://pdbe.org/5hrf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hrf RCSB], [https://www.ebi.ac.uk/pdbsum/5hrf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hrf ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5hrf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hrf OCA], [http://pdbe.org/5hrf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hrf RCSB], [http://www.ebi.ac.uk/pdbsum/5hrf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hrf ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DPOLX_ASFB7 DPOLX_ASFB7] Error-prone polymerase lacking a proofreading 3'-5' exonuclease which plays a role in viral DNA repair. Specifically binds intermediates in the single-nucleotide base-excision repair process. Also catalyzes DNA polymerization with low nucleotide-insertion fidelity. Together with the viral DNA ligase, fills the single nucleotide gaps generated by the AP endonuclease.<ref>PMID:12595253</ref> <ref>PMID:11685239</ref>
==See Also==
*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Asf]]
[[Category: African swine fever virus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Chen, Y Q]]
[[Category: Synthetic construct]]
[[Category: Gan, J H]]
[[Category: Chen YQ]]
[[Category: Zhang, J]]
[[Category: Gan JH]]
[[Category: Asfv]]
[[Category: Zhang J]]
[[Category: Polx]]
[[Category: Transferase-dna complex]]

Latest revision as of 13:51, 16 August 2023

The crystal structure of AsfvPolX: DNA5: dGTP ternary complexThe crystal structure of AsfvPolX: DNA5: dGTP ternary complex

Structural highlights

5hrf is a 4 chain structure with sequence from African swine fever virus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.25Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DPOLX_ASFB7 Error-prone polymerase lacking a proofreading 3'-5' exonuclease which plays a role in viral DNA repair. Specifically binds intermediates in the single-nucleotide base-excision repair process. Also catalyzes DNA polymerization with low nucleotide-insertion fidelity. Together with the viral DNA ligase, fills the single nucleotide gaps generated by the AP endonuclease.[1] [2]

See Also

References

  1. ↑ Garcia-Escudero R, Garcia-Diaz M, Salas ML, Blanco L, Salas J. DNA polymerase X of African swine fever virus: insertion fidelity on gapped DNA substrates and AP lyase activity support a role in base excision repair of viral DNA. J Mol Biol. 2003 Mar 7;326(5):1403-12. PMID:12595253
  2. ↑ Showalter AK, Byeon IJ, Su MI, Tsai MD. Solution structure of a viral DNA polymerase X and evidence for a mutagenic function. Nat Struct Biol. 2001 Nov;8(11):942-6. PMID:11685239 doi:10.1038/nsb1101-942

5hrf, resolution 2.25Å

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