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==Acoustic injectors for drop-on-demand serial femtosecond crystallography==
==Acoustic injectors for drop-on-demand serial femtosecond crystallography==
<StructureSection load='5hl4' size='340' side='right' caption='[[5hl4]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='5hl4' size='340' side='right'caption='[[5hl4]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5hl4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ensifer_meliloti Ensifer meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HL4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5HL4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5hl4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti_1021 Sinorhizobium meliloti 1021]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HL4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HL4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f81|5f81]], [[5f80|5f80]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SMa0751 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266834 Ensifer meliloti])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hl4 OCA], [https://pdbe.org/5hl4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hl4 RCSB], [https://www.ebi.ac.uk/pdbsum/5hl4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hl4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5hl4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hl4 OCA], [http://pdbe.org/5hl4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5hl4 RCSB], [http://www.ebi.ac.uk/pdbsum/5hl4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5hl4 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q92ZP9_RHIME Q92ZP9_RHIME]
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 5hl4" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 5hl4" style="background-color:#fffaf0;"></div>
==See Also==
*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ensifer meliloti]]
[[Category: Large Structures]]
[[Category: Agarwal, R]]
[[Category: Sinorhizobium meliloti 1021]]
[[Category: Allaire, M]]
[[Category: Agarwal R]]
[[Category: Alonso-Mori, R]]
[[Category: Allaire M]]
[[Category: Andi, B]]
[[Category: Alonso-Mori R]]
[[Category: Bachega, J F.R]]
[[Category: Andi B]]
[[Category: Bommer, M]]
[[Category: Bachega JFR]]
[[Category: Brewster, A S]]
[[Category: Bommer M]]
[[Category: Browne, M C]]
[[Category: Brewster AS]]
[[Category: Chatterjee, R]]
[[Category: Browne MC]]
[[Category: Cho, E]]
[[Category: Chatterjee R]]
[[Category: Cohen, A E]]
[[Category: Cho E]]
[[Category: Cowan, M]]
[[Category: Cohen AE]]
[[Category: Datwani, S]]
[[Category: Cowan M]]
[[Category: Davidson, V L]]
[[Category: Datwani S]]
[[Category: Defever, J]]
[[Category: Davidson VL]]
[[Category: Eaton, B]]
[[Category: Defever J]]
[[Category: Ellson, R]]
[[Category: Eaton B]]
[[Category: Feng, Y]]
[[Category: Ellson R]]
[[Category: Ghislain, L P]]
[[Category: Feng Y]]
[[Category: Glownia, J M]]
[[Category: Ghislain LP]]
[[Category: Han, G]]
[[Category: Glownia JM]]
[[Category: Hattne, J]]
[[Category: Han G]]
[[Category: Hellmich, J]]
[[Category: Hattne J]]
[[Category: Heroux, A]]
[[Category: Hellmich J]]
[[Category: Ibrahim, M]]
[[Category: Heroux A]]
[[Category: Kern, J]]
[[Category: Ibrahim M]]
[[Category: Kuczewski, A]]
[[Category: Kern J]]
[[Category: Lemke, H T]]
[[Category: Kuczewski A]]
[[Category: Liu, P]]
[[Category: Lemke HT]]
[[Category: Majlof, L]]
[[Category: Liu P]]
[[Category: McClintock, W M]]
[[Category: Majlof L]]
[[Category: Myers, S]]
[[Category: McClintock WM]]
[[Category: Nelsen, S]]
[[Category: Myers S]]
[[Category: Olechno, J]]
[[Category: Nelsen S]]
[[Category: Orville, A M]]
[[Category: Olechno J]]
[[Category: Roessler, C G]]
[[Category: Orville AM]]
[[Category: Sauter, N K]]
[[Category: Roessler CG]]
[[Category: Soares, A S]]
[[Category: Sauter NK]]
[[Category: Soltis, M S]]
[[Category: Soares AS]]
[[Category: Song, H]]
[[Category: Soltis MS]]
[[Category: Stearns, R G]]
[[Category: Song H]]
[[Category: Tran, R]]
[[Category: Stearns RG]]
[[Category: Tsai, Y]]
[[Category: Tran R]]
[[Category: Uervirojnangkoorn, M]]
[[Category: Tsai Y]]
[[Category: Wilmot, C M]]
[[Category: Uervirojnangkoorn M]]
[[Category: Yachandra, V]]
[[Category: Wilmot CM]]
[[Category: Yano, J]]
[[Category: Yachandra V]]
[[Category: Yukl, E T]]
[[Category: Yano J]]
[[Category: Zhu, D]]
[[Category: Yukl ET]]
[[Category: Zouni, A]]
[[Category: Zhu D]]
[[Category: Oxidoreductase]]
[[Category: Zouni A]]
[[Category: Ring-hydroxylating dioxygenase]]

Latest revision as of 13:45, 16 August 2023

Acoustic injectors for drop-on-demand serial femtosecond crystallographyAcoustic injectors for drop-on-demand serial femtosecond crystallography

Structural highlights

5hl4 is a 1 chain structure with sequence from Sinorhizobium meliloti 1021. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.2Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q92ZP9_RHIME

Publication Abstract from PubMed

X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable for macromolecular crystallography. Each X-ray pulse typically lasts for tens of femtoseconds and the interval between pulses is many orders of magnitude longer. Here we describe two novel acoustic injection systems that use focused sound waves to eject picoliter to nanoliter crystal-containing droplets out of microplates and into the X-ray pulse from which diffraction data are collected. The on-demand droplet delivery is synchronized to the XFEL pulse scheme, resulting in X-ray pulses intersecting up to 88% of the droplets. We tested several types of samples in a range of crystallization conditions, wherein the overall crystal hit ratio (e.g., fraction of images with observable diffraction patterns) is a function of the microcrystal slurry concentration. We report crystal structures from lysozyme, thermolysin, and stachydrine demethylase (Stc2). Additional samples were screened to demonstrate that these methods can be applied to rare samples.

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography.,Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Heroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A Structure. 2016 Apr 5;24(4):631-40. doi: 10.1016/j.str.2016.02.007. Epub 2016 Mar, 17. PMID:26996959[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Heroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A. Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Structure. 2016 Apr 5;24(4):631-40. doi: 10.1016/j.str.2016.02.007. Epub 2016 Mar, 17. PMID:26996959 doi:http://dx.doi.org/10.1016/j.str.2016.02.007

5hl4, resolution 2.20Å

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