1m2t: Difference between revisions

Revision as of 00:33, 3 May 2008

Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution

OverviewOverview

The crystal structure of the ribosome-inactivating protein (RIP) mistletoe lectin I (ML-I) from Viscum album in complex with adenine has been refined to 1.9 A resolution. High quality crystals of the ML-I complex were obtained by the method of vapour diffusion using the high density protein crystal growth system (HDPCG) on the international space station, mission ISS 6A. Hexagonal crystals were grown during three months under microgravity conditions. Diffraction data to 1.9A were collected applying synchrotron radiation and cryo- techniques. The structure was refined subsequently to analyse the structure of ML-I and particularly the active site conformation, complexed by adenine that mimics the RNA substrate binding.

About this StructureAbout this Structure

1M2T is a Protein complex structure of sequences from Viscum album. Full crystallographic information is available from OCA.

ReferenceReference

Crystallisation under microgravity of mistletoe lectin I from Viscum album with adenine monophosphate and the crystal structure at 1.9 A resolution., Krauspenhaar R, Rypniewski W, Kalkura N, Moore K, DeLucas L, Stoeva S, Mikhailov A, Voelter W, Betzel Ch, Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1704-7. Epub, 2002 Sep 26. PMID:12351890 Page seeded by OCA on Sat May 3 00:33:54 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1m2t.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1m2t", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADE:ADENINE'>ADE</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
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-|DOMAIN=
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-|RELATEDENTRY=[[2mll|2MLL]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m2t OCA], [http://www.ebi.ac.uk/pdbsum/1m2t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m2t RCSB]</span>
-}}
 '''Mistletoe Lectin I from Viscum album in Complex with Adenine Monophosphate. Crystal Structure at 1.9 A Resolution'''
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 [[Category: Protein complex]]
 [[Category: Viscum album]]
-[[Category: rRNA N-glycosylase]]
+[[Category: RRNA N-glycosylase]]
 [[Category: Betzel, C.]]
 [[Category: DeLucas, L.]]
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 [[Category: Stoeva, S.]]
 [[Category: Voelter, W.]]
-[[Category: ribosome inactivation]]
+[[Category: Ribosome inactivation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:33:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:10:33 2008''