1ijf: Difference between revisions
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<StructureSection load='1ijf' size='340' side='right'caption='[[1ijf]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='1ijf' size='340' side='right'caption='[[1ijf]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1ijf]] is a 2 chain structure with sequence from | <table><tr><td colspan='2'>[[1ijf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IJF FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ijf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ijf OCA], [https://pdbe.org/1ijf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ijf RCSB], [https://www.ebi.ac.uk/pdbsum/1ijf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ijf ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ijf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ijf OCA], [https://pdbe.org/1ijf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ijf RCSB], [https://www.ebi.ac.uk/pdbsum/1ijf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ijf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/EF1A_YEAST EF1A_YEAST] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Andersen | [[Category: Andersen GR]] | ||
[[Category: Kinzy | [[Category: Kinzy TG]] | ||
[[Category: Nyborg | [[Category: Nyborg J]] | ||
[[Category: Pedersen | [[Category: Pedersen L]] | ||
[[Category: Valente | [[Category: Valente L]] | ||
Latest revision as of 11:34, 16 August 2023
Nucleotide exchange mechanisms in the eEF1A-eEF1Ba complexNucleotide exchange mechanisms in the eEF1A-eEF1Ba complex
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedIn the elongation cycle of protein biosynthesis, the nucleotide exchange factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved eEF1A-eEF1Balpha structure, we determined the structures of the eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha are consistent with in vivo data. The base, sugar and alpha-phosphate bind as in other known nucleotide G-protein complexes, whereas the beta- and gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that inserts into the Mg2+ binding site of eEF1A is lethal. This together with the structures emphasizes the essential role of Mg2+ in nucleotide exchange in the eEF1A-eEF1Balpha complex. Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex.,Andersen GR, Valente L, Pedersen L, Kinzy TG, Nyborg J Nat Struct Biol. 2001 Jun;8(6):531-4. PMID:11373622[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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