8hkk: Difference between revisions
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The entry | ==ion channel== | ||
<StructureSection load='8hkk' size='340' side='right'caption='[[8hkk]], [[Resolution|resolution]] 2.84Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8hkk]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HKK FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.84Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hkk OCA], [https://pdbe.org/8hkk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hkk RCSB], [https://www.ebi.ac.uk/pdbsum/8hkk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hkk ProSAT]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[https://www.uniprot.org/uniprot/KCNT1_HUMAN KCNT1_HUMAN] Malignant migrating focal seizures of infancy;Autosomal dominant nocturnal frontal lobe epilepsy. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry. | |||
== Function == | |||
[https://www.uniprot.org/uniprot/KCNT1_HUMAN KCNT1_HUMAN] Outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro) (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The sodium-activated Slo2.2 channel is abundantly expressed in the brain, playing a critical role in regulating neuronal excitability. The Na(+)-binding site and the underlying mechanisms of Na(+)-dependent activation remain unclear. Here, we present cryoelectron microscopy (cryo-EM) structures of human Slo2.2 in closed, open, and inhibitor-bound form at resolutions of 2.6-3.2 A, revealing gating mechanisms of Slo2.2 regulation by cations and a potent inhibitor. The cytoplasmic gating ring domain of the closed Slo2.2 harbors multiple K(+) and Zn(2+) sites, which stabilize the channel in the closed conformation. The open Slo2.2 structure reveals at least two Na(+)-sensitive sites where Na(+) binding induces expansion and rotation of the gating ring that opens the inner gate. Furthermore, a potent inhibitor wedges into a pocket formed by pore helix and S6 helix and blocks the pore. Together, our results provide a comprehensive structural framework for the investigation of Slo2.2 channel gating, Na(+) sensation, and inhibition. | |||
Structural basis of human Slo2.2 channel gating and modulation.,Zhang J, Liu S, Fan J, Yan R, Huang B, Zhou F, Yuan T, Gong J, Huang Z, Jiang D Cell Rep. 2023 Jul 25;42(8):112858. doi: 10.1016/j.celrep.2023.112858. PMID:37494189<ref>PMID:37494189</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 8hkk" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | |||
[[Category: Large Structures]] | |||
[[Category: Jiang DH]] | |||
[[Category: Zhang JT]] | |||
Latest revision as of 11:25, 16 August 2023
ion channelion channel
Structural highlights
DiseaseKCNT1_HUMAN Malignant migrating focal seizures of infancy;Autosomal dominant nocturnal frontal lobe epilepsy. The disease is caused by variants affecting the gene represented in this entry. The disease is caused by variants affecting the gene represented in this entry. FunctionKCNT1_HUMAN Outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro) (By similarity). Publication Abstract from PubMedThe sodium-activated Slo2.2 channel is abundantly expressed in the brain, playing a critical role in regulating neuronal excitability. The Na(+)-binding site and the underlying mechanisms of Na(+)-dependent activation remain unclear. Here, we present cryoelectron microscopy (cryo-EM) structures of human Slo2.2 in closed, open, and inhibitor-bound form at resolutions of 2.6-3.2 A, revealing gating mechanisms of Slo2.2 regulation by cations and a potent inhibitor. The cytoplasmic gating ring domain of the closed Slo2.2 harbors multiple K(+) and Zn(2+) sites, which stabilize the channel in the closed conformation. The open Slo2.2 structure reveals at least two Na(+)-sensitive sites where Na(+) binding induces expansion and rotation of the gating ring that opens the inner gate. Furthermore, a potent inhibitor wedges into a pocket formed by pore helix and S6 helix and blocks the pore. Together, our results provide a comprehensive structural framework for the investigation of Slo2.2 channel gating, Na(+) sensation, and inhibition. Structural basis of human Slo2.2 channel gating and modulation.,Zhang J, Liu S, Fan J, Yan R, Huang B, Zhou F, Yuan T, Gong J, Huang Z, Jiang D Cell Rep. 2023 Jul 25;42(8):112858. doi: 10.1016/j.celrep.2023.112858. PMID:37494189[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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