1lz8: Difference between revisions

Revision as of 00:26, 3 May 2008

LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES

OverviewOverview

A general method for solving the phase problem from native crystals of macromolecules has long eluded structural biology. For well diffracting crystals this goal can now be achieved, as is shown here, thanks to modern data collection techniques and new statistical phasing algorithms. Using solely a native crystal of tetragonal hen egg-white lysozyme, a protein of 14 kDa molecular mass, it was possible to detect the positions of the ten sulfur and seven chlorine atoms from their anomalous signal, and proceed from there to obtain an electron-density map of very high quality.

About this StructureAbout this Structure

1LZ8 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Can anomalous signal of sulfur become a tool for solving protein crystal structures?, Dauter Z, Dauter M, de La Fortelle E, Bricogne G, Sheldrick GM, J Mol Biol. 1999 May 28;289(1):83-92. PMID:10339407 Page seeded by OCA on Sat May 3 00:26:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1lz8.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1lz8 |SIZE=350|CAPTION= <scene name='initialview01'>1lz8</scene>, resolution 1.53&Aring;
+The line below this paragraph, containing "STRUCTURE_1lz8", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1lz8|  PDB=1lz8  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lz8 OCA], [http://www.ebi.ac.uk/pdbsum/1lz8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lz8 RCSB]</span>
-}}
 '''LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES'''
@@ Line 31: / Line 28: @@
 [[Category: Fortelle, E De La.]]
 [[Category: Sheldrick, G M.]]
-[[Category: glycosidase]]
+[[Category: Glycosidase]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: o-glycosyl]]
+[[Category: O-glycosyl]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:26:44 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:09:11 2008''