5h8d: Difference between revisions

From Proteopedia
Jump to navigation Jump to search
← Older edit
No edit summary
No edit summary
 
Line 3: Line 3:
<StructureSection load='5h8d' size='340' side='right'caption='[[5h8d]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
<StructureSection load='5h8d' size='340' side='right'caption='[[5h8d]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5h8d]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H8D OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5H8D FirstGlance]. <br>
<table><tr><td colspan='2'>[[5h8d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5H8D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5H8D FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5h8o|5h8o]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5h8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h8d OCA], [http://pdbe.org/5h8d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5h8d RCSB], [http://www.ebi.ac.uk/pdbsum/5h8d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5h8d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5h8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5h8d OCA], [https://pdbe.org/5h8d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5h8d RCSB], [https://www.ebi.ac.uk/pdbsum/5h8d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5h8d ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
Line 19: Line 19:
==See Also==
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Camelus glama]]
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lu, A]]
[[Category: Lu A]]
[[Category: Ploegh, H L]]
[[Category: Ploegh HL]]
[[Category: Ruan, J]]
[[Category: Ruan J]]
[[Category: Schmidt, F I]]
[[Category: Schmidt FI]]
[[Category: Tang, C]]
[[Category: Tang C]]
[[Category: Wu, H]]
[[Category: Wu H]]
[[Category: Antibody fragment]]
[[Category: Asc-binding]]
[[Category: Immune system]]
[[Category: Inflammasome]]
[[Category: Vhh nanobody]]

Latest revision as of 10:27, 9 August 2023

Crystal structure of an ASC binding nanobodyCrystal structure of an ASC binding nanobody

Structural highlights

5h8d is a 1 chain structure with sequence from Lama glama. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.89Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Myeloid cells assemble inflammasomes in response to infection or cell damage; cytosolic sensors activate pro-caspase-1, indirectly for the most part, via the adaptors ASC and NLRC4. This leads to secretion of proinflammatory cytokines and pyroptosis. To explore complex formation under physiological conditions, we generated an alpaca single domain antibody, VHHASC, which specifically recognizes the CARD of human ASC via its type II interface. VHHASC not only impairs ASC(CARD) interactions in vitro, but also inhibits inflammasome activation in response to NLRP3, AIM2, and NAIP triggers when expressed in living cells, highlighting a role of ASC in all three types of inflammasomes. VHHASC leaves the Pyrin domain of ASC functional and stabilizes a filamentous intermediate of inflammasome activation. Incorporation of VHHASC-EGFP into these structures allowed the visualization of endogenous ASC(PYD) filaments for the first time. These data revealed that cross-linking of ASC(PYD) filaments via ASC(CARD) mediates the assembly of ASC foci.

A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly.,Schmidt FI, Lu A, Chen JW, Ruan J, Tang C, Wu H, Ploegh HL J Exp Med. 2016 May 2;213(5):771-90. doi: 10.1084/jem.20151790. Epub 2016 Apr 11. PMID:27069117[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Schmidt FI, Lu A, Chen JW, Ruan J, Tang C, Wu H, Ploegh HL. A single domain antibody fragment that recognizes the adaptor ASC defines the role of ASC domains in inflammasome assembly. J Exp Med. 2016 May 2;213(5):771-90. doi: 10.1084/jem.20151790. Epub 2016 Apr 11. PMID:27069117 doi:http://dx.doi.org/10.1084/jem.20151790

5h8d, resolution 1.89Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=5h8d&oldid=3825174"