1lva: Difference between revisions

Revision as of 00:19, 3 May 2008

Crystal structure of a C-terminal fragment of Moorella thermoacetica elongation factor SelB

OverviewOverview

SelB is an elongation factor needed for the co-translational incorporation of selenocysteine. Selenocysteine is coded by a UGA stop codon in combination with a specific downstream mRNA hairpin. In bacteria, the C-terminal part of SelB recognizes this hairpin, while the N-terminal part binds GTP and tRNA in analogy with elongation factor Tu (EF-Tu). We present the crystal structure of a C-terminal fragment of SelB (SelB-C) from Moorella thermoacetica at 2.12 A resolution, solved by a combination of selenium and yttrium multiwavelength anomalous dispersion. This 264 amino acid fragment contains the entire C-terminal extension beginning after the EF-Tu-homologous domains. SelB-C consists of four similar winged-helix domains arranged into the shape of an L. This is the first example of winged-helix domains involved in RNA binding. The location of conserved basic amino acids, together with data from the literature, define the position of the mRNA-binding site. Steric requirements indicate that a conformational change may occur upon ribosome interaction. Structural observations and data in the literature suggest that this change happens upon mRNA binding.

About this StructureAbout this Structure

1LVA is a Single protein structure of sequence from Moorella thermoacetica. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of an mRNA-binding fragment of Moorella thermoacetica elongation factor SelB., Selmer M, Su XD, EMBO J. 2002 Aug 1;21(15):4145-53. PMID:12145214 Page seeded by OCA on Sat May 3 00:19:50 2008

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OCA

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 [[Image:1lva.gif|left|200px]]
- {{Structure
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-|GENE= SelB(amino acids 370-634) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1525 Moorella thermoacetica])
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-}}
 '''Crystal structure of a C-terminal fragment of Moorella thermoacetica elongation factor SelB'''
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 [[Category: Selmer, M.]]
 [[Category: Su, X D.]]
-[[Category: winged-helix]]
+[[Category: Winged-helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:19:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:07:47 2008''