1rh6: Difference between revisions
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<StructureSection load='1rh6' size='340' side='right'caption='[[1rh6]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='1rh6' size='340' side='right'caption='[[1rh6]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[1rh6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RH6 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [https://pdbe.org/1rh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rh6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rh6 OCA], [https://pdbe.org/1rh6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rh6 RCSB], [https://www.ebi.ac.uk/pdbsum/1rh6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rh6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/VXIS_LAMBD VXIS_LAMBD] Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Escherichia virus Lambda]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cascio | [[Category: Cascio D]] | ||
[[Category: Clubb | [[Category: Clubb RT]] | ||
[[Category: Johnson | [[Category: Johnson RC]] | ||
[[Category: Sam | [[Category: Sam MD]] | ||
Revision as of 14:24, 2 August 2023
Bacteriophage Lambda Excisionase (Xis)-DNA ComplexBacteriophage Lambda Excisionase (Xis)-DNA Complex
Structural highlights
FunctionVXIS_LAMBD Excisionase and integrase are necessary for the excision of prophage from the host genome by site-specific recombination at the att site. Publication Abstract from PubMedThe excisionase (Xis) protein from bacteriophage lambda is the best characterized member of a large family of recombination directionality factors that control integrase-mediated DNA rearrangements. It triggers phage excision by cooperatively binding to sites X1 and X2 within the phage, bending DNA significantly and recruiting the phage-encoded integrase (Int) protein to site P2. We have determined the co-crystal structure of Xis with its X2 DNA-binding site at 1.7A resolution. Xis forms a unique winged-helix motif that interacts with the major and minor grooves of its binding site using an alpha-helix and an ordered beta-hairpin (wing), respectively. Recognition is achieved through an elaborate water-mediated hydrogen-bonding network at the major groove interface, while the preformed hairpin forms largely non-specific interactions with the minor groove. The structure of the complex provides insights into how Xis recruits Int cooperatively, and suggests a plausible mechanism by which it may distort longer DNA fragments significantly. It reveals a surface on the protein that is likely to mediate Xis-Xis interactions required for its cooperative binding to DNA. Crystal structure of the excisionase-DNA complex from bacteriophage lambda.,Sam MD, Cascio D, Johnson RC, Clubb RT J Mol Biol. 2004 Apr 23;338(2):229-40. PMID:15066428[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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