1lok: Difference between revisions

Revision as of 00:07, 3 May 2008

The 1.20 Angstrom Resolution Crystal Structure of the Aminopeptidase from Aeromonas proteolytica Complexed with Tris: A Tale of Buffer Inhibition

OverviewOverview

The aminopeptidase from Aeromonas proteolytica (AAP) is a bridged bimetallic enzyme that removes the N-terminal amino acid from a peptide chain. To fully understand the metal roles in the reaction pathway of AAP we have solved the 1.20 A resolution crystal structure of native AAP (PDB ID = 1LOK). The high-quality electron density maps showed a single Tris molecule chelated to the active site Zn(2+), alternate side chain conformations for some side chains, a sodium ion that mediates a crystal contact, a surface thiocyanate ion, and several potential hydrogen atoms. In addition, the high precision of the atomic positions has led to insight into the protonation states of some of the active site amino acid side chains.

About this StructureAbout this Structure

1LOK is a Single protein structure of sequence from Vibrio proteolyticus. Full crystallographic information is available from OCA.

ReferenceReference

The 1.20 A resolution crystal structure of the aminopeptidase from Aeromonas proteolytica complexed with tris: a tale of buffer inhibition., Desmarais WT, Bienvenue DL, Bzymek KP, Holz RC, Petsko GA, Ringe D, Structure. 2002 Aug;10(8):1063-72. PMID:12176384 Page seeded by OCA on Sat May 3 00:07:19 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1lok.jpg|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Bacterial_leucyl_aminopeptidase Bacterial leucyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.10 3.4.11.10] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lok OCA], [http://www.ebi.ac.uk/pdbsum/1lok PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lok RCSB]</span>
-}}
 '''The 1.20 Angstrom Resolution Crystal Structure of the Aminopeptidase from Aeromonas proteolytica Complexed with Tris: A Tale of Buffer Inhibition'''
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 [[Category: Petsko, G A.]]
 [[Category: Ringe, D.]]
-[[Category: aminopeptidase]]
+[[Category: Aminopeptidase]]
-[[Category: high resolution]]
+[[Category: High resolution]]
-[[Category: mechanism]]
+[[Category: Mechanism]]
-[[Category: metal coordination]]
+[[Category: Metal coordination]]
-[[Category: metalloenzyme]]
+[[Category: Metalloenzyme]]
-[[Category: tri]]
+[[Category: Tri]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:07:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:05:21 2008''